Maggio 21, 2024

Mycobacterium avium subsp. paratuberculosis an infection utilizing host biomarker-based ELISAs significantly

A reference measurement of circulating ATPase inhibitory issue 1 (IF1) in people by LC-MS/MS: Comparability with standard ELISA

ATPase inhibitory issue 1 (IF1) is a 9.5 kDa protein that binds to mitochondrial and plasma membrane ATP synthase and selectively inhibits ATP hydrolysis. Lately, IF1 was recognized in systemic circulation in people. IF1 appeared as an impartial determinant of HDL-cholesterol with decrease ranges in coronary coronary heart illness (CHD) sufferers. Furthermore, IF1 was additionally discovered to negatively affiliate with mortality in these sufferers, supporting the notion that circulating IF1 could possibly be a promising biomarker of heart problems. Nonetheless, in earlier research, IF1 was quantified by a non-standardized aggressive enzyme-linked immunosorbent assay (ELISA). 

  • Herein, we’ve validated a liquid chromatography-tandem mass spectrometry methodology (LC-MS/MS) enabling the correct quantification of IF1 in human plasma.
  • Plasma IF1 was trypsin-digested by way of an optimized process earlier than LC-MS/MS evaluation. The tactic was efficiently validated over four impartial experiments into the vary of 100-1500 ng/mL. Intra- and inter-assay variation coefficients had by no means exceeded 14.2% and accuracy ranged between 95% and 102% for the chosen EAGGAFGK peptide marker.
  • Subsequently, the outcomes of the LC-MS/MS methodology have been in contrast with these obtained utilizing ELISA in 204 people from the GENES research.
  • We discovered that IF1 plasma ranges obtained utilizing each strategies have been strongly correlated (r = 0.89, p < 0.0001), whereas the Bland-Altman plot didn’t point out any main statistically important variations. To clinically validate LC-MS/MS, we confirmed the constructive correlation between IF1 plasma ranges and HDL-cholesterol (r = 0.38, p < 0.0001).
  • Apart from, we discovered decrease IF1 plasma ranges in CHD sufferers in comparison with controls (431 ± 132 ng/mL and 555 ± 173 ng/mL, respectively; p < 0.0001). Therefore, it may be concluded that the introduced LC-MS/MS analytical methodology offers a extremely particular technique for IF1 quantification in human plasma and could possibly be proposed as a reference methodology.

VN1R5 Antibody

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VN1R5 Antibody

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VN1R5 Antibody

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EUR 1205

VN1R5 Antibody

MBS7119588-005mg 0.05mg
EUR 150

VN1R5 Antibody

MBS7119588-01mg 0.1mg
EUR 190

VN1R5 Antibody

MBS7119588-5x01mg 5x0.1mg
EUR 845

VN1R5 Antibody

MBS856611-01mg 0.1mg
EUR 305

VN1R5 Antibody

MBS856611-01mLAF405L 0.1mL(AF405L)
EUR 465

VN1R5 Antibody

MBS856611-01mLAF405S 0.1mL(AF405S)
EUR 465

VN1R5 Antibody

MBS856611-01mLAF610 0.1mL(AF610)
EUR 465

VN1R5 Antibody

MBS856611-01mLAF635 0.1mL(AF635)
EUR 465

VN1R5 Antibody

MBS9214122-01mL 0.1mL
EUR 415

VN1R5 Antibody

MBS9214122-5x01mL 5x0.1mL
EUR 1841

VN1R5 Antibody

MBS9508342-005mL 0.05mL
EUR 285

VN1R5 Antibody

MBS9508342-01mL 0.1mL
EUR 385

VN1R5 Antibody

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EUR 1590

VN1R5 Antibody (HRP)

20-abx308444
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VN1R5 Antibody (HRP)

abx308444-100g 100 µg
EUR 362.5

VN1R5 Antibody (HRP)

abx308444-20g 20 µg
EUR 162.5

VN1R5 Antibody (HRP)

abx308444-50g 50 µg
EUR 250

VN1R5 Antibody (FITC)

20-abx308445
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VN1R5 Antibody (FITC)

abx308445-100g 100 µg
EUR 362.5

VN1R5 Antibody (FITC)

abx308445-20g 20 µg
EUR 162.5

VN1R5 Antibody (FITC)

abx308445-50g 50 µg
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VN1R5 Antibody (Biotin)

20-abx308446
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VN1R5 Antibody (Biotin)

abx308446-100g 100 µg
EUR 362.5

VN1R5 Antibody (Biotin)

abx308446-20g 20 µg
EUR 162.5

VN1R5 Antibody (Biotin)

abx308446-50g 50 µg
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Polyclonal VN1R5 Antibody

APR13971G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human VN1R5 . This antibody is tested and proven to work in the following applications:

VN1R5 Polyclonal Antibody

A65978
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VN1R5 Antibody - middle region

MBS3219025-01mL 0.1mL
EUR 455

VN1R5 Antibody - middle region

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EUR 1995

VN1R5 Antibody, HRP conjugated

1-CSB-PA801813LB01HU
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Description: A polyclonal antibody against VN1R5. Recognizes VN1R5 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

VN1R5 Antibody; HRP conjugated

MBS7005322-005mg 0.05mg
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VN1R5 Antibody; HRP conjugated

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VN1R5 Antibody, FITC conjugated

1-CSB-PA801813LC01HU
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Description: A polyclonal antibody against VN1R5. Recognizes VN1R5 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

VN1R5 Antibody; FITC conjugated

MBS7005153-005mg 0.05mg
EUR 190

VN1R5 Antibody; FITC conjugated

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VN1R5 Antibody, Biotin conjugated

1-CSB-PA801813LD01HU
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Description: A polyclonal antibody against VN1R5. Recognizes VN1R5 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

OACA05248-100UG - VN1R5 Antibody

OACA05248-100UG 100ug
EUR 319

OAAB18586-100UL - VN1R5 Antibody

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OAAF05244-100UG - VN1R5 Antibody

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VN1R5 Antibody; Biotin conjugated

MBS7001919-005mg 0.05mg
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VN1R5 Antibody; Biotin conjugated

MBS7001919-01mg 0.1mg
EUR 270

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VN1R5 Polyclonal Antibody, HRP Conjugated

A65979
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VN1R5 Polyclonal Antibody, FITC Conjugated

A65980
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VN1R5 Polyclonal Antibody, Biotin Conjugated

A65981
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ARP71965_P050-25UL - VN1R5 Antibody - middle region

ARP71965_P050-25UL 25ul
EUR 99

OAAB13777-400UL - VN1R5 Antibody - middle region

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ARP71965_P050 - VN1R5 Antibody - middle region (ARP71965_P050)

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VN1R5

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Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx030146-400ul 400 ul
EUR 627.6

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx030146-80l 80 µl
EUR 343.2

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

20-abx015491
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Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

20-abx302011
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Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx433448-200ul 200 ul
EUR 343.2

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx433449-200ul 200 ul
EUR 343.2

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

20-abx322836
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  • 100 ug
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Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx302011-100g 100 µg
EUR 362.5

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx302011-20g 20 µg
EUR 162.5

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx302011-50g 50 µg
EUR 250

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx030146-400l 400 µl
EUR 518.75

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx015491-1mg 1 mg
EUR 43.75

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx433448-100g 100 µg
EUR 250

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx433449-100g 100 µg
EUR 250

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx322836-100g 100 µg
EUR 250

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx322836-50g 50 µg
EUR 187.5

Polyclonal VN1R5 (aa109-119) Antibody (internal region)

APR13969G 0.1 mg
EUR 580.8
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human VN1R5 (aa109-119) (internal region). This antibody is tested and proven to work in the following applications:

Polyclonal VN1R5 (aa347-356) Antibody (internal region)

APR13970G 0.1 mg
EUR 580.8
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human VN1R5 (aa347-356) (internal region). This antibody is tested and proven to work in the following applications:

VN1R5 siRNA

20-abx939465
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VN1R5 Peptide

42-689P 0.1 mg
EUR 405.6
Description: (IN) VN1R5 Peptide

VN1R5 Peptide

42-690P 0.1 mg
EUR 405.6
Description: (IN) VN1R5 Peptide

VN1R5 cDNA Clone

MBS1272734-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
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EUR 855

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VN1R5 siRNA (Human)

MBS8240682-30nmol 30nmol
EUR 565

Detection of latent types of Mycobacterium avium subsp. paratuberculosis an infection utilizing host biomarker-based ELISAs significantly improves paratuberculosis diagnostic sensitivity

Bovine paratuberculosis (PTB) is a power granulomatous enteritis, brought on by Mycobacterium avium subsp. paratuberculosis (MAP), accountable for necessary financial losses within the dairy trade. Present diagnostic strategies have low sensitivities for detection of latent types of MAP an infection, outlined by focal granulomatous lesions and scarce humoral response or MAP presence. In distinction, patent infections correspond to multifocal and diffuse sorts of enteritis the place there’s elevated antibody manufacturing, and substantial mycobacterial load.

Our earlier RNA-Seq evaluation allowed the number of 5 candidate biomarkers overexpressed in peripheral blood of MAP contaminated Holstein cows with focal (ABCA13 and MMP8) and diffuse (FAM84A, SPARC and DES) lesions vs. management animals with no detectable PTB-associated lesions in gut and regional lymph nodes. The goal of the present research was to evaluate the PTB diagnostic potential of business ELISAs designed for the particular detection of those biomarkers.

The flexibility of those ELISAs to establish animals with latent and/or patent types of MAP an infection was investigated utilizing serum from naturally contaminated cattle (n = 88) and non-infected management animals (n = 67). ROC evaluation revealed that the ABCA13-based ELISA confirmed the very best diagnostic accuracy for the detection of contaminated animals with focal lesions (AUC 0.837, sensitivity 79.25% and specificity 88.06%) and with any kind of histological lesion (AUC 0.793, sensitivity 69.41% and specificity 86.57%) enhancing on the diagnostic efficiency of the favored IDEXX ELISA and different standard diagnostic strategies.

SPARC and MMP8 confirmed the very best diagnostic accuracy for the detection of animals with multifocal (AUC 0.852) and diffuse lesions (AUC 0.831), respectively. In conclusion, our outcomes recommend that quantification of ABCA13, SPARC and MMP8 by ELISA has the potential for implementation as a diagnostic software to reliably establish MAP an infection, significantly enhancing early detection of MAP latent infections when antibody responses and fecal shedding are undetectable utilizing standard diagnostic strategies.

 

VN1R5 Antibody

MBS7006404-005mg 0.05mg
EUR 190

VN1R5 Antibody

MBS7006404-01mg 0.1mg
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VN1R5 Antibody

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EUR 1205

VN1R5 Antibody

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EUR 150

VN1R5 Antibody

MBS7119588-01mg 0.1mg
EUR 190

VN1R5 Antibody

MBS7119588-5x01mg 5x0.1mg
EUR 845

VN1R5 Antibody

MBS856611-01mg 0.1mg
EUR 305

VN1R5 Antibody

MBS856611-01mLAF405L 0.1mL(AF405L)
EUR 465

VN1R5 Antibody

MBS856611-01mLAF405S 0.1mL(AF405S)
EUR 465

VN1R5 Antibody

MBS856611-01mLAF610 0.1mL(AF610)
EUR 465

VN1R5 Antibody

MBS856611-01mLAF635 0.1mL(AF635)
EUR 465

VN1R5 Antibody

MBS9214122-01mL 0.1mL
EUR 415

VN1R5 Antibody

MBS9214122-5x01mL 5x0.1mL
EUR 1841

VN1R5 Antibody

MBS9508342-005mL 0.05mL
EUR 285

VN1R5 Antibody

MBS9508342-01mL 0.1mL
EUR 385

VN1R5 Antibody

MBS9508342-5x01mL 5x0.1mL
EUR 1590

VN1R5 Antibody (HRP)

20-abx308444
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VN1R5 Antibody (HRP)

abx308444-100g 100 µg
EUR 362.5

VN1R5 Antibody (HRP)

abx308444-20g 20 µg
EUR 162.5

VN1R5 Antibody (HRP)

abx308444-50g 50 µg
EUR 250

VN1R5 Antibody (FITC)

20-abx308445
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VN1R5 Antibody (FITC)

abx308445-100g 100 µg
EUR 362.5

VN1R5 Antibody (FITC)

abx308445-20g 20 µg
EUR 162.5

VN1R5 Antibody (FITC)

abx308445-50g 50 µg
EUR 250

VN1R5 Antibody (Biotin)

20-abx308446
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VN1R5 Antibody (Biotin)

abx308446-100g 100 µg
EUR 362.5

VN1R5 Antibody (Biotin)

abx308446-20g 20 µg
EUR 162.5

VN1R5 Antibody (Biotin)

abx308446-50g 50 µg
EUR 250

VN1R5 Antibody (Center)

MBS9210427-008mL 0.08mL
EUR 210

VN1R5 Antibody (Center)

MBS9210427-04mL 0.4mL
EUR 430

VN1R5 Antibody (Center)

MBS9210427-5x04mL 5x0.4mL
EUR 1910

Polyclonal VN1R5 Antibody

APR13971G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human VN1R5 . This antibody is tested and proven to work in the following applications:

VN1R5 Polyclonal Antibody

A65978
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  • 100 µg
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VN1R5 Antibody - middle region

MBS3219025-01mL 0.1mL
EUR 455

VN1R5 Antibody - middle region

MBS3219025-5x01mL 5x0.1mL
EUR 1995

VN1R5 Antibody, HRP conjugated

1-CSB-PA801813LB01HU
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  • 100ug
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Description: A polyclonal antibody against VN1R5. Recognizes VN1R5 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

VN1R5 Antibody; HRP conjugated

MBS7005322-005mg 0.05mg
EUR 190

VN1R5 Antibody; HRP conjugated

MBS7005322-01mg 0.1mg
EUR 270

VN1R5 Antibody; HRP conjugated

MBS7005322-5x01mg 5x0.1mg
EUR 1205

VN1R5 Antibody, FITC conjugated

1-CSB-PA801813LC01HU
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  • 100ug
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Description: A polyclonal antibody against VN1R5. Recognizes VN1R5 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

VN1R5 Antibody; FITC conjugated

MBS7005153-005mg 0.05mg
EUR 190

VN1R5 Antibody; FITC conjugated

MBS7005153-01mg 0.1mg
EUR 270

VN1R5 Antibody; FITC conjugated

MBS7005153-5x01mg 5x0.1mg
EUR 1205

OACA05248-50UG - VN1R5 Antibody

OACA05248-50UG 50ug
EUR 179

VN1R5 Antibody, Biotin conjugated

1-CSB-PA801813LD01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against VN1R5. Recognizes VN1R5 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

OACA05248-100UG - VN1R5 Antibody

OACA05248-100UG 100ug
EUR 319

OAAB18586-100UL - VN1R5 Antibody

OAAB18586-100UL 100ul
EUR 389

OAAF05244-100UG - VN1R5 Antibody

OAAF05244-100UG 100ug
EUR 329

VN1R5 Antibody; Biotin conjugated

MBS7001919-005mg 0.05mg
EUR 190

VN1R5 Antibody; Biotin conjugated

MBS7001919-01mg 0.1mg
EUR 270

VN1R5 Antibody; Biotin conjugated

MBS7001919-5x01mg 5x0.1mg
EUR 1205

VN1R5 Antibody (Center) Blocking Peptide

MBS9223287-INQUIRE INQUIRE Ask for price

VN1R5 Polyclonal Antibody, HRP Conjugated

A65979
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  • 100 µg
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VN1R5 Polyclonal Antibody, FITC Conjugated

A65980
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  • 100 µg
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  • 100 ul

VN1R5 Polyclonal Antibody, Biotin Conjugated

A65981
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  • 100 µg
  • 50 ul
  • 100 ul

ARP71965_P050-25UL - VN1R5 Antibody - middle region

ARP71965_P050-25UL 25ul
EUR 99

OAAB13777-400UL - VN1R5 Antibody - middle region

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EUR 389

ARP71965_P050 - VN1R5 Antibody - middle region (ARP71965_P050)

ARP71965_P050 100ul
EUR 389

VN1R5

CSB-CL801813HU 10 μg plasmid + 200μl Glycerol Ask for price

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx030146-400ul 400 ul
EUR 627.6

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx030146-80l 80 µl
EUR 343.2

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

20-abx015491
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  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

20-abx302011
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx433448-200ul 200 ul
EUR 343.2

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx433449-200ul 200 ul
EUR 343.2

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

20-abx322836
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  • 100 ug
  • 50 ug

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx302011-100g 100 µg
EUR 362.5

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx302011-20g 20 µg
EUR 162.5

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx302011-50g 50 µg
EUR 250

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx030146-400l 400 µl
EUR 518.75

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx015491-1mg 1 mg
EUR 43.75

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx433448-100g 100 µg
EUR 250

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx433449-100g 100 µg
EUR 250

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx322836-100g 100 µg
EUR 250

Vomeronasal 1 Receptor 5 (gene/pseudogene) (VN1R5) Antibody

abx322836-50g 50 µg
EUR 187.5

Polyclonal VN1R5 (aa109-119) Antibody (internal region)

APR13969G 0.1 mg
EUR 580.8
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human VN1R5 (aa109-119) (internal region). This antibody is tested and proven to work in the following applications:

Polyclonal VN1R5 (aa347-356) Antibody (internal region)

APR13970G 0.1 mg
EUR 580.8
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human VN1R5 (aa347-356) (internal region). This antibody is tested and proven to work in the following applications:

VN1R5 siRNA

20-abx939465
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

VN1R5 Peptide

42-689P 0.1 mg
EUR 405.6
Description: (IN) VN1R5 Peptide

VN1R5 Peptide

42-690P 0.1 mg
EUR 405.6
Description: (IN) VN1R5 Peptide

VN1R5 cDNA Clone

MBS1272734-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

VN1R5 cDNA Clone

MBS1272734-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

VN1R5 siRNA (Human)

MBS8240682-15nmol 15nmol
EUR 405

VN1R5 siRNA (Human)

MBS8240682-30nmol 30nmol
EUR 565
 

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