Giugno 17, 2024

Inibizione della metilazione del DNA da parte di agenti cancerogeni chimici in vitro

Il ruolo dell’eta DNA polimerasi nello spettro delle mutazioni UV nelle cellule umane.Nell’uomo, l’inattivazione del gene della DNA polimerasi eta (pol eta) provoca sensibilità alla luce solare e provoca la variante incline al cancro xeroderma pigmentoso (XP-V). Le cellule degli individui XP-V hanno una ridotta capacità di replicare il DNA danneggiato dalle radiazioni UV e mostrano ipermutabilità dopo l’esposizione alle radiazioni UV.

  • I test biochimici hanno dimostrato la capacità della trama di bypassare   i dimeri cis-syn-ciclobutano della timina  ,   il danno al DNA più comune generato dai raggi UV. Nella maggior parte dei casi, questa soluzione è impeccabile. Per determinare l’effettiva domanda di campo in vivo, le cellule XP-V (XP30RO) sono state integrate con il gene della poleta di tipo selvaggio.
  • Abbiamo utilizzato due   cloni corretti sul campo  per studiare la caratterizzazione in vivo delle mutazioni prodotte dalle DNA polimerasi durante la sintesi del DNA di vettori navetta irradiati con UV trasfettati in cellule ospiti umane che erano o non erano state precedentemente esposte a radiazioni UV.
  • Il rifornimento funzionale delle cellule XP-V da parte dei grafici ha ridotto la frequenza di mutazione nelle coppie di basi CG e TA e ripristinato la mutagenesi UV a livelli normali. L’irradiazione UV delle cellule ospiti prima della trasfezione ha notevolmente aumentato la frequenza di mutazione nei vettori intatti e, inoltre, specialmente nelle cellule XP30RO carenti di pulci nei siti 5′-TT dei plasmidi irradiati con UV.
  • Questi risultati dimostrano chiaramente il ruolo protettivo della trama contro i cambiamenti indotti dalla radiazione UV e l’attivazione di processi mutageni indipendenti dalla trama da parte degli UV.

Clonazione molecolare del gene di riparazione umano mediante escissione del nucleotide ERCC4.

  1. ERCC4 è stato precedentemente identificato negli ibridi di cellule somatiche come un gene umano che corregge una carenza di riparazione dell’escissione nucleotidica nelle cellule di criceto mutanti. La strategia di clonazione ERCC4  prevedeva la trasfettazione della linea cellulare di criceto carente di riparazione UV41 con una libreria cosmidica umana sCos-1 derivata dal cromosoma 16.
  2. È stata osservata una maggiore resistenza ai raggi UV con un trasformante dalla libreria dei cosmidi e due trasformanti secondari UV41. I cloni cosmidi che  ospitano un gene ERCC4 funzionale sono stati isolati dalla libreria trasformante secondaria mediante selezione in Escherichia coli per esprimere il gene di resistenza alla neomicina fuso che era presente nel vettore sCos-1.
  3. Cosmidi mappati su 16p13.13-p13.2, una posizione assegnata a ERCC4, utilizzando ibridi di cellule somatiche. Dopo la trasfezione in UV41, i sei   cloni cosmidi  hanno fornito correzioni parziali che vanno dal 30% al 64%, sebbene tutti sembrassero contenere l’intero gene  . La capacità di asportare in vitro   i dimeri  di timina    da un plasmide mediante estratti cellulari trasformanti è qualitativamente correlata con l’aumentata resistenza alle radiazioni UV.

Effetto delle radiazioni ultraviolette (UV) A, UVB o ionizzanti sul ciclo cellulare delle cellule di melanoma umano.

  • Uno dei componenti importanti di una risposta cellulare alle radiazioni è l’attivazione dei checkpoint del ciclo cellulare. È noto che sia la radiazione ultravioletta (UV) che quella ionizzante (IR) possono attivare checkpoint nel passaggio dalla fase G (1) alla fase S, da G (2) alla mitosi e durante la replicazione del DNA.
  • Valutazione dell’impatto dell’irradiazione con diverse lunghezze d’onda sulle variazioni del ciclo cellulare.
  • Le cellule di melanoma IPC-298 carenti di p53 sono state irradiate con 10 J cm (-2) UVA, 40 mJ cm (-2) UVB o 7,5 Gy IR. Gli effetti del ciclo cellulare sono stati quindi determinati mediante citometria a flusso di DNA/5-bromodeossiuridina a due parametri.
  • Le cellule IPC-298 irradiate in G (1) con UVA non sono state arrestate alla transizione G (1) / S, ma alla transizione G (2) / M. Nonostante la carenza di p53, le cellule hanno mostrato l’arresto di G (1) dopo l’esposizione ai raggi UVB. Inoltre, IR non ha influenzato la fase G (1) o S, ma ha causato un arresto della fase G (2). Quindi, l’effetto degli UVA, ma non degli UVB, sul ciclo cellulare nelle cellule di melanoma carenti di p53 è paragonabile a quello dell’IR.
  • UVA e IR inducono rotture del filamento e danni al DNA mediati dai radicali e UVB essenzialmente induce   dimeri  di timina    che portano a rotture del filamento associate alla riparazione dell’escissione. Diversi effetti del ciclo cellulare possono essere una conseguenza di diversi tipi di danno al DNA. I risultati hanno mostrato che le cellule carenti di p53 irradiate da UVB vengono mantenute in G (1). L’irradiazione con la componente solare della radiazione UVB può quindi ritardare vantaggiosamente la crescita del tumore nella pelle umana che porta   cloni cellulari  con la mutazione p53.

Fotochimica catalizzatrice dei desossiribozimi: fotoriparazione   di dimeri  di timina   dipendenti e indipendenti dal cofattore.

  • Le strategie sperimentali di selezione in vitro progettate per testare la validità dell ‘”ipotesi del mondo dell’RNA” hanno mostrato una gamma catalitica molto più ampia per l’RNA (e gli acidi nucleici in generale) rispetto ai ribozimi naturali.
  • Volevamo indagare se le reazioni fotochimiche potessero essere catalizzate dagli enzimi dell’acido nucleico. Sono stati effettuati esperimenti di selezione in vitro per ottenere deossiribozimi “fotoliasi” in grado di fotorevertire   i dimeri  di ciclobutano timina  in presenza del cofattore serotonina.
  • Durante la selezione in vitro dalla   timina  –  un dimero   contenente una libreria di DNA casuale irradiata con una luce > 300 nm, sono comparsi due pool di molecole di nuclei catalitici – uno che richiedeva serotonina per l’attività e l’altro, sorprendentemente, no. La caratterizzazione  dei cloni indipendenti dalla serotonina ha mostrato che la lunghezza d’onda ottimale per la loro attività di riparazione (circa 1400 volte) è di circa 300 nm, in particolare spostata verso il rosso dal massimo assorbimento del solo DNA.

Una vasta gamma di agenti cancerogeni chimici finali ha inibito il trasferimento di gruppi metilici dalla S-adenosilmetionina al DNA emimetilato in una reazione catalizzata dalla metiltransferasi della milza di topo.

  1. La formazione di siti labili alcalini nel DNA ha ridotto la sua capacità di accettare gruppi metilici in vitro, ma la reazione di metilazione era molto meno sensibile ai  dimeri  di timina    o alle rotture del doppio filamento  . Gli agenti cancerogeni hanno indotto la formazione di danno al DNA labile alcalino, ma il grado osservato di inibizione della metiltransferasi era superiore a quello previsto per questo danno da solo.
  2. Alcuni agenti cancerogeni sono stati anche in grado di modificare e inattivare direttamente l’enzima metiltransferasi. Il trattamento con benzo(a)pirene su cellule BALB / 3T3 A31 vive dal   clone   1-13, ma non con cellule C3H / 10T1 / 2   clone   8, ha comportato una diminuzione del 12% del contenuto totale di 5-metilcitosina nel DNA cellulare .

Thymine

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Thymine

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Thymine

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Lenti ORF clone of Human thymine-DNA glycosylase (TDG), mGFP tagged

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Thymine DNA glycosylase (TDG) mouse monoclonal antibody, clone AT2F7, Purified

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Thymine DNA glycosylase (TDG) mouse monoclonal antibody, clone AT2F7, Purified

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Thymine-d4

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Lenti ORF clone of Human thymine-DNA glycosylase (TDG), Myc-DDK-tagged

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Lenti ORF clone of Human thymine-DNA glycosylase (TDG), Myc-DDK-tagged

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3`UTR clone of thymine-DNA glycosylase (TDG) for miRNA target validation

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5,6-Dihydro Thymine

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5,6-Dihydro Thymine

MBS6030663-1g MyBiosource 1(g 500 EUR

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Lenti ORF clone of Tdg (Myc-DDK-tagged ORF) - Rat thymine-DNA glycosylase (Tdg), (10 ug)

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Thymine (5-Methyluracil)

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Thymine (5-Methyluracil)

MBS657825-1kg MyBiosource 1kg 610 EUR

Thymine (5-Methyluracil)

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MBS657825-5x1kg MyBiosource 5x1kg 2650 EUR

Lenti ORF clone of Tdg (mGFP-tagged) - Mouse thymine DNA glycosylase (Tdg), transcript variant 2

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5,6-Dihydro Thymine-d6

D449442 Toronto Research Chemicals 1mg 2916 EUR

TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase)

MBS6001824-02mL MyBiosource 0.2(mL 695 EUR

TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase)

MBS6001824-5x02mL MyBiosource 5x0.2mL 2975 EUR

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Lenti ORF clone of Tdg (Myc-DDK-tagged) - Mouse thymine DNA glycosylase (Tdg), transcript variant 2

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TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (FITC)

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TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (HRP)

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TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (APC)

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TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (FITC)

MBS6502352-02mL MyBiosource 0.2mL 980 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (FITC)

MBS6502352-5x02mL MyBiosource 5x0.2mL 4250 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (HRP)

MBS6502353-02mL MyBiosource 0.2mL 980 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (HRP)

MBS6502353-5x02mL MyBiosource 5x0.2mL 4250 EUR

Anti-Thymine Dimer mAb

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MBS6396237-01mL MyBiosource 0.1mL 920 EUR

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MBS6396237-5x01mL MyBiosource 5x0.1mL 3990 EUR

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (FITC)

MBS6396239-01mL MyBiosource 0.1mL 920 EUR

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (FITC)

MBS6396239-5x01mL MyBiosource 5x0.1mL 3990 EUR

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (HRP)

MBS6396240-01mL MyBiosource 0.1mL 920 EUR

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (HRP)

MBS6396240-5x01mL MyBiosource 5x0.1mL 3990 EUR

TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (Biotin)

MBS6502340-02mL MyBiosource 0.2mL 980 EUR

TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (Biotin)

MBS6502340-5x02mL MyBiosource 5x0.2mL 4250 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (Biotin)

MBS6502351-02mL MyBiosource 0.2mL 980 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (Biotin)

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Thymine DNA Glycosylase Antibody

E38PA3334 EnoGene 100ul 225 EUR

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MBS8581892-01mL MyBiosource 0.1mL 305 EUR

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MBS8581892-01mLAF405L MyBiosource 0.1mL(AF405L) 465 EUR

Thymine DNA Glycosylase Antibody

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Thymine DNA Glycosylase Antibody

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Thymine DNA Glycosylase Antibody

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TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (Biotin)

MBS6396238-01mL MyBiosource 0.1mL 920 EUR

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (Biotin)

MBS6396238-5x01mL MyBiosource 5x0.1mL 3990 EUR

Thymine-​2-​13C-​1,​3-​15N2

T412149 Toronto Research Chemicals 100mg 661 EUR

TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 405)

MBS6502343-01mL MyBiosource 0.1mL 980 EUR

TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 405)

MBS6502343-5x01mL MyBiosource 5x0.1mL 4250 EUR

TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 490)

MBS6502344-01mL MyBiosource 0.1mL 980 EUR

TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 490)

MBS6502344-5x01mL MyBiosource 5x0.1mL 4250 EUR

TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 550)

MBS6502345-01mL MyBiosource 0.1mL 980 EUR

TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 550)

MBS6502345-5x01mL MyBiosource 5x0.1mL 4250 EUR

TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 650)

MBS6502346-01mL MyBiosource 0.1mL 980 EUR

TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 650)

MBS6502346-5x01mL MyBiosource 5x0.1mL 4250 EUR

TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 750)

MBS6502347-01mL MyBiosource 0.1mL 980 EUR

TDG, CT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 750)

MBS6502347-5x01mL MyBiosource 5x0.1mL 4250 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 405)

MBS6502354-01mL MyBiosource 0.1mL 980 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 405)

MBS6502354-5x01mL MyBiosource 5x0.1mL 4250 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 490)

MBS6502355-01mL MyBiosource 0.1mL 980 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 490)

MBS6502355-5x01mL MyBiosource 5x0.1mL 4250 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 550)

MBS6502356-01mL MyBiosource 0.1mL 980 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 550)

MBS6502356-5x01mL MyBiosource 5x0.1mL 4250 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 650)

MBS6502357-01mL MyBiosource 0.1mL 980 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 650)

MBS6502357-5x01mL MyBiosource 5x0.1mL 4250 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 750)

MBS6502358-01mL MyBiosource 0.1mL 980 EUR

TDG, NT (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase) (MaxLight 750)

MBS6502358-5x01mL MyBiosource 5x0.1mL 4250 EUR

7'-O-DMT-morpholino thymine

HY-152973 MedChemExpress Get quote Ask for price

Thymine-DNA Glycosylase (Recombinant)

20-abx073364 Abbexa
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  • 1 mg
  • 20 ug
  • 5 ug

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (MaxLight 405)

MBS6396241-01mL MyBiosource 0.1mL 920 EUR

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (MaxLight 405)

MBS6396241-5x01mL MyBiosource 5x0.1mL 3990 EUR

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (MaxLight 490)

MBS6396242-01mL MyBiosource 0.1mL 920 EUR

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (MaxLight 490)

MBS6396242-5x01mL MyBiosource 5x0.1mL 3990 EUR

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (MaxLight 550)

MBS6396243-01mL MyBiosource 0.1mL 920 EUR

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (MaxLight 550)

MBS6396243-5x01mL MyBiosource 5x0.1mL 3990 EUR

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (MaxLight 650)

MBS6396244-01mL MyBiosource 0.1mL 920 EUR

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (MaxLight 650)

MBS6396244-5x01mL MyBiosource 5x0.1mL 3990 EUR

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (MaxLight 750)

MBS6396245-01mL MyBiosource 0.1mL 920 EUR

TDG (G/T Mismatch-specific Thymine DNA Glycosylase, Thymine-DNA Glycosylase, hTDG) (MaxLight 750)

MBS6396245-5x01mL MyBiosource 5x0.1mL 3990 EUR

Thymine DNA Glycosylase (TDG) Antibody

20-abx119073 Abbexa
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  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Thymine DNA Glycosylase (TDG) Antibody

20-abx134132 Abbexa
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  • 100 ul
  • 200 ul
  • 30 ul

Thymine DNA Glycosylase (TDG) Antibody

20-abx004405 Abbexa
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  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Thymine DNA Glycosylase (TDG) Antibody

20-abx001168 Abbexa
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  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Thymine DNA Glycosylase (TDG) Antibody

20-abx174778 Abbexa
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  • 1 mg
  • 200 ug

Thymine DNA Glycosylase (TDG) Antibody

20-abx178579 Abbexa
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  • 1 mg
  • 200 ug

Thymine DNA Glycosylase (TDG) Antibody

20-abx329663 Abbexa
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  • 100 ug
  • 50 ug

Thymine DNA Glycosylase (TDG) Antibody

abx238570-100ug Abbexa 100 ug 610.8 EUR

Thymine DNA Glycosylase (TDG) Antibody

20-abx322198 Abbexa
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  • 100 ul
  • 50 ul

Thymine DNA Glycosylase (TDG) Antibody

20-abx322224 Abbexa
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  • 100 ul
  • 50 ul

Thymine DNA Glycosylase (TDG) Antibody

abx119073-100l Abbexa 100 µl 312.5 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx119073-20l Abbexa 20 µl 43.75 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx119073-50l Abbexa 50 µl 237.5 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx238570-100l Abbexa 100 µl Ask for price

Thymine DNA Glycosylase (TDG) Antibody

abx238570-50l Abbexa 50 µl 350 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx322198-100g Abbexa 100 µg 350 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx322198-50g Abbexa 50 µg 237.5 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx329663-100g Abbexa 100 µg Ask for price

Thymine DNA Glycosylase (TDG) Antibody

abx329663-20g Abbexa 20 µg 187.5 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx329663-50g Abbexa 50 µg 250 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx322224-100g Abbexa 100 µg 350 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx322224-50g Abbexa 50 µg 237.5 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx004405-100l Abbexa 100 µl 400 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx004405-20l Abbexa 20 µl 175 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx004405-50l Abbexa 50 µl 275 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx001168-100l Abbexa 100 µl 400 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx001168-20l Abbexa 20 µl 175 EUR

Thymine DNA Glycosylase (TDG) Antibody

abx001168-50l Abbexa 50 µl 275 EUR

1-(alpha-L-Threofuranosyl)thymine

TNU1336-10mg TargetMol Chemicals 10mg Ask for price

1-(alpha-L-Threofuranosyl)thymine

TNU1336-1g TargetMol Chemicals 1g Ask for price

1-(alpha-L-Threofuranosyl)thymine

TNU1336-1mg TargetMol Chemicals 1mg Ask for price

1-(alpha-L-Threofuranosyl)thymine

TNU1336-50mg TargetMol Chemicals 50mg Ask for price

1-(alpha-L-Threofuranosyl)thymine

TNU1336-5mg TargetMol Chemicals 5mg Ask for price

Thymine 1-β-D-arabinofuranoside

HY-137848 MedChemExpress Get quote Ask for price

TDG Antibody / Thymine-DNA glycosylase

RQ7493 NSJ Bioreagents 100ug 300.3 EUR

Thymine-alpha,alpha,alpha,6-d4

T412157 Toronto Research Chemicals 10mg 64 EUR

Thymine-Dna Glycosylase (hTDG) Antibody

20-abx116089 Abbexa
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  • 150 ul
  • 50 ul

Thymine-DNA Glycosylase (hTDG) Antibody

20-abx137328 Abbexa
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  • 100 ug
  • 20 ug
  • 5 ug

Thymine-Dna Glycosylase (hTDG) Antibody

abx116089-100g Abbexa 100 µg Ask for price

Thymine-Dna Glycosylase (hTDG) Antibody

abx116089-10g Abbexa 10 µg 612.5 EUR

Thymine-Dna Glycosylase (hTDG) Antibody

abx116089-200g Abbexa 200 µg Ask for price

Thymine-DNA Glycosylase (hTDG) Antibody

abx137328-01mg Abbexa 0.1 mg 225 EUR

Thymine DNA Glycosylase Blocking Peptide

20-abx162196 Abbexa
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  • 1 mg
  • 5 mg

Thymine DNA Glycosylase Blocking Peptide

MBS8220703-1mg MyBiosource 1mg 190 EUR

Thymine DNA Glycosylase Blocking Peptide

MBS8220703-5mg MyBiosource 5mg 345 EUR

Thymine DNA Glycosylase Blocking Peptide

MBS8220703-5x5mg MyBiosource 5x5mg 1465 EUR

Rat Thymine DNA Glycosylase ELISA kit

E01A17217 BlueGene 96T 700 EUR

Rat Thymine DNA Glycosylase ELISA Kit

MBS049077-10x96StripWells MyBiosource 10x96-Strip-Wells 6725 EUR

Rat Thymine DNA Glycosylase ELISA Kit

MBS049077-48StripWells MyBiosource 48-Strip-Wells 550 EUR

Rat Thymine DNA Glycosylase ELISA Kit

MBS049077-5x96StripWells MyBiosource 5x96-Strip-Wells 3420 EUR

Rat Thymine DNA Glycosylase ELISA Kit

MBS049077-96StripWells MyBiosource 96-Strip-Wells 765 EUR

Cat Thymine DNA Glycosylase ELISA Kit

MBS079012-INQUIRE MyBiosource INQUIRE Ask for price

7’-OH-N-trityl morpholino thymine

TNU0641-10mg TargetMol Chemicals 10mg Ask for price

7’-OH-N-trityl morpholino thymine

TNU0641-1g TargetMol Chemicals 1g Ask for price

7’-OH-N-trityl morpholino thymine

TNU0641-1mg TargetMol Chemicals 1mg Ask for price

7’-OH-N-trityl morpholino thymine

TNU0641-50mg TargetMol Chemicals 50mg Ask for price

7’-OH-N-trityl morpholino thymine

TNU0641-5mg TargetMol Chemicals 5mg Ask for price

Rat Thymine DNA Glycosylase ELISA Kit

MBS7269712-10x96StripWells MyBiosource 10x96-Strip-Wells 5685 EUR

Rat Thymine DNA Glycosylase ELISA Kit

MBS7269712-48StripWells MyBiosource 48-Strip-Wells 485 EUR

Rat Thymine DNA Glycosylase ELISA Kit

MBS7269712-5x96StripWells MyBiosource 5x96-Strip-Wells 3020 EUR

Rat Thymine DNA Glycosylase ELISA Kit

MBS7269712-96StripWells MyBiosource 96-Strip-Wells 690 EUR

Rat Thymine DNA Glycosylase ELISA Kit

MBS1603811-10x96StripWells MyBiosource 10x96-Strip-Wells 3955 EUR

Rat Thymine DNA Glycosylase ELISA Kit

MBS1603811-48StripWells MyBiosource 48-Strip-Wells 305 EUR

Rat Thymine DNA Glycosylase ELISA Kit

MBS1603811-5x96StripWells MyBiosource 5x96-Strip-Wells 2005 EUR

Rat Thymine DNA Glycosylase ELISA Kit

MBS1603811-96StripWells MyBiosource 96-Strip-Wells 475 EUR

Goat Thymine DNA Glycosylase ELISA kit

E01A52116 BlueGene 96T 700 EUR

Duck Thymine DNA Glycosylase ELISA Kit

MBS096158-INQUIRE MyBiosource INQUIRE Ask for price

Fish Thymine DNA Glycosylase ELISA Kit

MBS090703-INQUIRE MyBiosource INQUIRE Ask for price

Goat Thymine DNA Glycosylase ELISA Kit

MBS080937-INQUIRE MyBiosource INQUIRE Ask for price

Cavy Thymine DNA Glycosylase ELISA Kit

MBS102584-INQUIRE MyBiosource INQUIRE Ask for price

Goat Thymine DNA Glycosylase ELISA Kit

MBS7271057-10x96StripWells MyBiosource 10x96-Strip-Wells 5685 EUR

Pertanto, gli agenti cancerogeni possono causare alterazioni ereditarie nei modelli di 5-metilcitosina in alcuni tipi di cellule mediante una varietà di meccanismi tra cui la formazione di addotti, l’induzione di siti apurina e le rotture di un singolo filamento e l’inattivazione diretta della DNA metiltransferasi.

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