by Confronto del legame cellulare e dell’assorbimento di fosfodiestere, fosforotioato e oligonucleotidi antisenso misti di fosforotioato e metilfosfonato. L’effetto delle modificazioni del fosforotioato (S-oligonucleotide) o del fosfodiestere fosforotioato terminale (SO-oligonucleotidi) o del fosfodiestere metilfosfonato (MP-O-oligonucleotidi) sul legame, assorbimento e degradazione delle cellule della milza murina è stato studiato utilizzando oligonucleotidi coniugati con fluoresceina ( FITC ) . Gli oligonucleotidi S hanno mostrato il legame e l’assorbimento cellulare più elevati seguiti dagli oligonucleotidi SO-, O- e MP-O-.
- Studi sulla concorrenza hanno dimostrato che gli S-oligonucleotidi hanno una maggiore affinità per i siti di legame degli oligonucleotidi di membrana poiché possono bloccare completamente il legame dell’O-oligonucleotidi con un rapporto molare di appena 0,1. L’assorbimento di tutti gli oligonucleotidi era maggiore nei linfociti B rispetto ai linfociti T ed era aumentato dalla stimolazione con il mitogeno dei linfociti B, il lipopolisaccaride.
- Sebbene le nostre cellule siano state purificate utilizzando tecniche convenzionali per eliminare le cellule morte, circa il 5% delle cellule è rimasto morto o morente come determinato dalla citometria a flusso utilizzando la colorazione con ioduro di propidio. Vale la pena notare che l’associazione degli oligonucleotidi con le cellule morte era circa 50 volte maggiore rispetto alle cellule viventi.
- La microscopia confocale ha confermato che gli oligonucleotidi nelle cellule vitali erano intracellulari e mostravano uno scarso assorbimento nel nucleo per 4 ore. Sebbene un’estesa degradazione degli O-oligonucleotidi intracellulari fosse visibile dopo 4 ore, non vi era alcuna degradazione rilevabile di S-, SO o MP-O-oligonucleotidi.
I cumuli di cellule mesangiali. Focolai nodulari di eccessiva crescita cellulare e matrice in coltura prolungata.
- Per studiare la capacità delle cellule mesangiali glomerulari (MC) di produrre matrice extracellulare, gli autori hanno studiato la MC in coltura utilizzando la microscopia ottica ed elettronica, nonché l’immunocitochimica. Le MC sono state ottenute da glomeruli di ratto isolati e mantenute per un massimo di 12 settimane in un mezzo contenente il 20% di siero fetale di vitello.
- La crescita eccessiva di MC dalla coltura primaria e fino a tre sottocolture ha mostrato un’organizzazione caratteristica costituita da filamenti di cellule allungate o stellate intrecciate. Dopo la confluenza dopo 10-16 giorni, le MC hanno continuato a crescere in multistrati irregolari. Gli MC hanno prodotto materiale di matrice extracellulare entro 2-4 giorni dopo la placcatura e grandi quantità di matrice si sono accumulate nel tempo. Dopo 2-3 settimane, focolai di eccessiva proliferazione di MC, secrezione di matrice e detriti cellulari necrotici formavano sporgenze nodulari che gradualmente formavano grandi collinette.
- Gli studi immunocitochimici di polloni MC sono stati eseguiti su piastre di coltura o su materiale asportato utilizzando specifici anticorpi policlonali di coniglio contro proteine della matrice isolate e secondi anticorpi coniugati con FITC e purificati per affinità .
- Durante i 3 giorni di coltivazione, le MC hanno sviluppato fibronectina e collagene di tipo I, III, IV e V. Nel tempo, i filamenti della matrice, soprattutto nella massa centrale delle colline, si sono colorati intensamente per questi componenti. La colorazione della laminina era meno pronunciata.
- La miosina delle cellule muscolari lisce è stata regolarmente trovata su fibre intracellulari discrete e nel materiale extracellulare delle collinette. La microscopia elettronica ha mostrato che le collinette erano costituite da cellule allungate sulla superficie e cellule stellate intervallate dalla matrice e dai detriti delle cellule necrotiche nel nucleo.
- I risultati mostrano che le MC proliferanti possono essere mantenute in colture omogenee per periodi più lunghi. Le MC producono grandi quantità di proteine della matrice extracellulare (collagene di tipo IV e V, fibronectina, laminina), che si trovano nei glomeruli normali.
- Le MC coltivate producono anche collagene interstiziale di tipo I e III. Le colline MC mostrano un accumulo di matrice nodulare simile a quello visto nel mesangio dei glomeruli malati. Si è concluso che un modello in vitro di crescita prolungata di MC potrebbe facilitare lo studio dei fattori che regolano la produzione della matrice mesangiale. Tale modello potrebbe essere utilizzato per studiare la risposta mesangiale a specifici stimoli infiammatori o metabolici.
PET / CT in vivo per i recettori EphB4 di piccoli animali utilizzando il peptide marcato con 64Cu.
- Molti tumori solidi sovraesprimono il recettore EphB4, un membro della famiglia del recettore dell’efrina tirosin-chinasi. L’imaging EphB4 non invasivo ha il potenziale per aumentare i tassi di diagnosi precoce, monitorare la risposta alla terapia anti-EphB4 e migliorare i risultati dei pazienti. Lo scopo di questo studio era di valutare un nuovo (64) peptide marcato con Cu con un’elevata affinità per il legame dei recettori PET ai recettori EphB4.
- TNYLFSPNGPIARAW Il peptide legante EphB4 (TNYL-RAW) è stato coniugato con isotiocianato di fluoresceina (FITC) e DOTA. DOTA-TNYL-RAW è stato etichettato con (64) Cu con un’elevata efficienza di etichettatura. L’ affinità di legame di TNYL-RAW e dei suoi derivati per EphB4 ricombinante purificato è stata determinata utilizzando la tecnologia di risonanza plasmonica di superficie. Il legame in vitro di FITC-TNYL-RAW e (64) Cu-DOTA-TNYL-RAW alle cellule tumorali è stato valutato mediante microscopia a fluorescenza e metodi di conteggio della radioattività. La biodistribuzione in vivo e la PET/TC in piccoli animali sono state eseguite in topi portatori di tumori CT26 e PC-3M che esprimono EphB4, nonché di tumori A549 negativi per EphB4.
- TNYL-RAW e suoi derivati hanno mostrato un’elevata affinità di legame a EphB4, con una costante di equilibrio di dissociazione di 1,98-23 nM. In vitro, sia FITC-TNYL-RAW che (64) Cu-DOTA-TNYL-RAW sono stati assorbiti selettivamente dalle cellule CT26 e PC-3M, ma non dalle cellule A549. Il legame di FITC-TNYL-RAW e (64) Cu-DOTA-TNYL-RAW alle cellule CT26 e PC-3M può essere bloccato dall’eccesso di TNYL-RAW. In vivo, (64) Cu-DOTA-TNYL-RAW ha mostrato un assorbimento significativamente maggiore nei tumori PC-3M rispetto ai tumori A549, con una dose percentuale iniettata per grammo di tumore pari a 0,84 ± 0,09 e 0,44 ± 0,09 24 ore dopo l’iniezione del radiotracciante , rispettivamente. La PET / CT in piccoli animali ha rivelato chiaramente (64) la deposizione di Cu-DOTA-TNYL-RAW nei tumori CT26 e PC-3M, ma non nei tumori A549. Inoltre, l’assorbimento (64) di Cu-DOTA-TNYL-RAW nei tumori CT26 e PC-3M può essere bloccato dal TNYL-RAW freddo.
- È stato progettato un sistema di espressione (pSKGFP) che consente l’espressione di frammenti variabili a catena singola come proteine di fusione con proteine fluorescenti verdi modificate. Questo sistema di espressione è paragonabile ai vettori di visualizzazione dei fagi comunemente usati e consente una caratterizzazione in un unico passaggio di anticorpi ricombinanti selezionati mediante citometria a flusso o colorazione cellulare fluorescente.
- Due diversi anticorpi a frammento variabile a catena singola, entrambi diretti contro Ralstonia solanacearum lipopolisaccaridi, sono stati geneticamente fusi con una proteina fluorescente verde spostata in rosso e la proteina di fusione risultante è stata testata per l’utilità.
- Questi fluocorpi possono essere prodotti in colture cellulari batteriche e purificati mediante cromatografia per affinità con metalli immobilizzati . Funzionano bene nella citometria a flusso e nella colorazione immunofluorescente delle cellule, sono specifici per i loro antigeni bersaglio e, a differenza degli anticorpi coniugati con FITC , non sbiadiscono quando irradiati.
Caratterizzazione dell’anticorpo monoclonale anti-H-2 e suo utilizzo nella purificazione dell’antigene su larga scala.
- È stato riscontrato che un anticorpo monoclonale (MAb) di ratto anti-topo, M1/42, reagisce con gli antigeni H-2 degli aplotipi a, b, d, j, k, siu (tutti gli aplotipi testati). Questo anticorpo, dopo essersi legato alle cellule e aver reagito con FITC – Ig anti-ratto coniugato , può essere utilizzato per quantificare l’espressione di H-2 su diversi tipi cellulari.
- L’anticorpo è stato anche utile per confrontare i prodotti H-2 precipitati da diversi aplotipi. Perline di sefarosio-4B accoppiate a M1/42 sono state utilizzate per purificare gli antigeni H-2d mediante cromatografia di affinità .
- Molecole di H-2 pure sono state eluite dalla colonna in DOC allo 0,5%, NaCl 0,65 M, Tris 20 mM, pH 8,0, producendo da 110 a 180 microgrammi di cellule tumorali H-2d / 10 (10) P815. Questo anticorpo, utilizzato in serie con MAb specifici per H-2Kk 11-4.1, ha consentito la purificazione di Dk e Dd rispettivamente dalle cellule RDM-4 e YAC.
c-myc Affinity Purified Antibody, FITC Conjugated |
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| MBS194206-5x1mg | MyBiosource | 5x1mg | 12370 EUR |
VSV-G Affinity Purified Antibody, FITC Conjugated |
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| MBS194388-INQUIRE | MyBiosource | INQUIRE | Ask for price |
glu-glu Affinity Purified Antibody, FITC Conjugated |
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| MBS194239-1mg | MyBiosource | 1mg | 2765 EUR |
glu-glu Affinity Purified Antibody, FITC Conjugated |
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| MBS194239-5x1mg | MyBiosource | 5x1mg | 12370 EUR |
Glutathione-S-Transferase (GST) Affinity Purified Antibody, FITC Conjugated |
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| MBS194246-01mg | MyBiosource | 0.1mg | 305 EUR |
Glutathione-S-Transferase (GST) Affinity Purified Antibody, FITC Conjugated |
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| MBS194246-5x01mg | MyBiosource | 5x0.1mg | 1305 EUR |
HA Affinity Purified Antibody, HRP Conjugated |
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| MBS194257-01mg | MyBiosource | 0.1mg | 390 EUR |
HA Affinity Purified Antibody, HRP Conjugated |
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| MBS194258-01mg | MyBiosource | 0.1mg | 560 EUR |
HA Affinity Purified Antibody, HRP Conjugated |
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| MBS194258-5x01mg | MyBiosource | 5x0.1mg | 2520 EUR |
T7 Affinity Purified Antibody, HRP Conjugated |
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| MBS194364-INQUIRE | MyBiosource | INQUIRE | Ask for price |
T7 Affinity Purified Antibody, HRP Conjugated |
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| MBS194365-01mg | MyBiosource | 0.1mg | 570 EUR |
T7 Affinity Purified Antibody, HRP Conjugated |
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| MBS194365-5x01mg | MyBiosource | 5x0.1mg | 2565 EUR |
V5 Affinity Purified Antibody, HRP Conjugated |
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| MBS194380-1mg | MyBiosource | 1mg | 3050 EUR |
V5 Affinity Purified Antibody, HRP Conjugated |
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| MBS194380-5x1mg | MyBiosource | 5x1mg | 13665 EUR |
V5 Affinity Purified Antibody, HRP Conjugated |
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| MBS194381-01mg | MyBiosource | 0.1mg | 560 EUR |
V5 Affinity Purified Antibody, HRP Conjugated |
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| MBS194381-5x01mg | MyBiosource | 5x0.1mg | 2520 EUR |
AU1 Affinity Purified Antibody, HRP Conjugated |
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| MBS194166-1mg | MyBiosource | 1mg | 4350 EUR |
AU1 Affinity Purified Antibody, HRP Conjugated |
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| MBS194166-5x1mg | MyBiosource | 5x1mg | 19515 EUR |
ECS Affinity Purified Antibody, HRP Conjugated |
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| MBS194225-01mg | MyBiosource | 0.1mg | 560 EUR |
ECS Affinity Purified Antibody, HRP Conjugated |
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| MBS194225-5x01mg | MyBiosource | 5x0.1mg | 2520 EUR |
ECS Affinity Purified Antibody, HRP Conjugated |
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| MBS194226-1mg | MyBiosource | 1mg | 4350 EUR |
ECS Affinity Purified Antibody, HRP Conjugated |
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| MBS194226-5x1mg | MyBiosource | 5x1mg | 19515 EUR |
KT3 Affinity Purified Antibody, HRP Conjugated |
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| MBS194293-1mg | MyBiosource | 1mg | 4350 EUR |
KT3 Affinity Purified Antibody, HRP Conjugated |
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| MBS194293-5x1mg | MyBiosource | 5x1mg | 19515 EUR |
6-His Affinity Purified Antibody, HRP Conjugated |
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| MBS194007-01mg | MyBiosource | 0.1mg | 570 EUR |
6-His Affinity Purified Antibody, HRP Conjugated |
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| MBS194007-5x01mg | MyBiosource | 5x0.1mg | 2565 EUR |
c-myc Affinity Purified Antibody, HRP Conjugated |
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| MBS194207-01mg | MyBiosource | 0.1mg | 570 EUR |
c-myc Affinity Purified Antibody, HRP Conjugated |
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| MBS194207-5x01mg | MyBiosource | 5x0.1mg | 2565 EUR |
c-myc Affinity Purified Antibody, HRP Conjugated |
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| MBS194208-01mg | MyBiosource | 0.1mg | 560 EUR |
c-myc Affinity Purified Antibody, HRP Conjugated |
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| MBS194208-5x01mg | MyBiosource | 5x0.1mg | 2520 EUR |
VSV-G Affinity Purified Antibody, HRP Conjugated |
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| MBS194389-INQUIRE | MyBiosource | INQUIRE | Ask for price |
6-His Affinity Purified Antibody, HRP Conjugated |
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| MBS194394-01mg | MyBiosource | 0.1mg | 570 EUR |
6-His Affinity Purified Antibody, HRP Conjugated |
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| MBS194394-5x01mg | MyBiosource | 5x0.1mg | 2565 EUR |
glu-glu Affinity Purified Antibody, HRP Conjugated |
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| MBS194240-1mg | MyBiosource | 1mg | 4350 EUR |
glu-glu Affinity Purified Antibody, HRP Conjugated |
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| MBS194240-5x1mg | MyBiosource | 5x1mg | 19515 EUR |
glu-glu Affinity Purified Antibody, HRP Conjugated |
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| MBS194241-1mg | MyBiosource | 1mg | 4350 EUR |
glu-glu Affinity Purified Antibody, HRP Conjugated |
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| MBS194241-5x1mg | MyBiosource | 5x1mg | 19515 EUR |
Cy2 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| MBS417864-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy5 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| MBS417865-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy3 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| MBS417873-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy2 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| SC015 | ABM | 250 µg | 145 EUR |
Cy5 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| SC016 | ABM | 250 µg | 145 EUR |
Cy3 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| SC025 | ABM | 250 µg | 145 EUR |
Biotin Conjugated Affinity Purified anti-Mouse Kappa [Rabbit] |
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| GWB-10B679 | GenWay Biotech | 1 mg | Ask for price |
Cy2 Conjugated Affinity Purified anti-Human IgG [H&L] [Goat] |
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| MBS417858-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy5 Conjugated Affinity Purified anti-Human IgG [H&L] [Goat] |
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| MBS417859-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy5 Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat] |
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| MBS417861-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy3 Conjugated Affinity Purified anti-Human IgG [H&L] [Goat] |
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| MBS417869-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy3 Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat] |
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| MBS417871-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy5 Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat] |
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| SC012 | ABM | 250 µg | 145 EUR |
Cy3 Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat] |
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| SC023 | ABM | 250 µg | 145 EUR |
Cy2 Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey] |
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| MBS417851-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy5 Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey] |
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| MBS417853-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy2 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| MBS417862-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy5 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| MBS417863-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy3 Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey] |
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| MBS417866-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy3 Conjugated Affinity Purified anti-Rabbit IgG (H&L) [Goat] |
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| MBS417872-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy2 Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey] |
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| SC002 | ABM | 250 µg | 145 EUR |
Cy5 Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey] |
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| SC004 | ABM | 250 µg | 145 EUR |
Cy2 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| SC013 | ABM | 250 µg | 145 EUR |
Cy5 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| SC014 | ABM | 250 µg | 145 EUR |
Cy3 Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey] |
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| SC017 | ABM | 250 µg | 145 EUR |
Cy3 Conjugated Affinity Purified anti-Rabbit IgG (H&L) [Goat] |
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| SC024 | ABM | 250 µg | 145 EUR |
Cy2 Conjugated Affinity Purified anti-Chicken IgG [H&L] [Goat] |
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| MBS417850-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy2 Conjugated Affinity Purified anti-Hamster IgG [H&L] [Goat] |
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| MBS417854-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy5 Conjugated Affinity Purified anti-Hamster IgG [H&L] [Goat] |
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| MBS417855-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy2 Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey] |
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| MBS417856-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy5 Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey] |
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| MBS417857-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy2 Conjugated Affinity Purified anti-Mouse IgG [H&L] [Donkey] |
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| MBS417860-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy3 Conjugated Affinity Purified anti-Hamster IgG [H&L] [Goat] |
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| MBS417867-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy3 Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey] |
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| MBS417868-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy5 Conjugated Affinity Purified anti-Hamster IgG [H&L] [Goat] |
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| SC006 | ABM | 250 µg | 145 EUR |
Cy2 Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey] |
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| SC007 | ABM | 250 µg | 145 EUR |
Cy5 Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey] |
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| SC008 | ABM | 250 µg | 145 EUR |
Cy2 Conjugated Affinity Purified anti-Mouse IgG [H&L] [Donkey] |
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| SC011 | ABM | 250 µg | 145 EUR |
Cy3 Conjugated Affinity Purified anti-Hamster IgG [H&L] [Goat] |
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| SC019 | ABM | 250 µg | 145 EUR |
Cy3 Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey] |
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| SC020 | ABM | 250 µg | 145 EUR |
Biotin Conjugated Affinity Purified Anti-RAT IgG F(c) (RABBIT) |
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| GWB-B4EC30 | GenWay Biotech | 1.5 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (GOAT) |
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| GWB-4AAFE1 | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (GOAT) |
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| GWB-327A5B | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT) |
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| GWB-460460 | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-MOUSE IgG F(c) (GOAT) |
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| GWB-175D9B | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-HUMAN IgM (Fc5u) (RABBIT) |
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| GWB-B1AFAF | GenWay Biotech | 1.5 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT) |
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| GWB-E14E58 | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-RABBIT IgG F(c) (GOAT) |
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| GWB-FEFD15 | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-GOAT IgG F(c) (RABBIT) |
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| GWB-50BF10 | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-RABBIT IgG (H&L) (GOAT) |
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| GWB-16F495 | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-HUMAN (kappa chain) (GOAT) |
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| GWB-77D4DD | GenWay Biotech | 1 mg | Ask for price |
Texas Red Conjugated Affinity Purified anti-Cat IgG [H&L] [Goat] |
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| MBS417984-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Dog IgG [H&L] [Goat] |
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| MBS417988-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| MBS418009-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Cat IgG [H&L] [Goat] |
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| ST003 | ABM | 250 µg | 125 EUR |
Texas Red Conjugated Affinity Purified anti-Dog IgG [H&L] [Goat] |
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| ST007 | ABM | 250 µg | 125 EUR |
Texas Red Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| ST028 | ABM | 250 µg | 125 EUR |
DyLight 488 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| MBS417885-01mg | MyBiosource | 0.1mg | 350 EUR |
DyLight 549 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| MBS417886-01mg | MyBiosource | 0.1mg | 350 EUR |
DyLight 488 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| SD011 | ABM | 100 µg | 200 EUR |
DyLight 549 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| SD012 | ABM | 100 µg | 200 EUR |
Biotin Conjugated Affinity Purified Anti-SHEEP IgG F(c) (RABBIT) |
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| GWB-A03546 | GenWay Biotech | 1.5 mg | Ask for price |
Biotin Conjugated Affinity Purified anti-Mouse IgG F(c) [Rabbit] |
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| GWB-D9ED60 | GenWay Biotech | 1.5 mg | Ask for price |
Biotin Conjugated Affinity Purified anti-Mouse IgG [H&L] [Rabbit] |
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| GWB-EAA016 | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-HUMAN IgG F(c) (RABBIT) |
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| GWB-5D1187 | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-SHEEP IgG (H&L) (RABBIT) |
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| GWB-1C237F | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (RABBIT) |
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| GWB-4515A4 | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-RAT-IgG (H&L) (RABBIT) |
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| GWB-832C22 | GenWay Biotech | 2 mg | Ask for price |
Peroxidase Conjugated Affinity Purified anti-Cat IgG [H&L] [Goat] |
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| MBS417941-025mg | MyBiosource | 0.25mg | 275 EUR |
Peroxidase Conjugated Affinity Purified anti-Dog IgG [H&L] [Goat] |
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| MBS417944-025mg | MyBiosource | 0.25mg | 275 EUR |
Peroxidase Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| MBS417965-025mg | MyBiosource | 0.25mg | 275 EUR |
Peroxidase Conjugated Affinity Purified anti-Cat IgG [H&L] [Goat] |
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| SH004 | ABM | 250 µg | 125 EUR |
Peroxidase Conjugated Affinity Purified anti-Dog IgG [H&L] [Goat] |
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| SH007 | ABM | 250 µg | 125 EUR |
Peroxidase Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| SH028 | ABM | 250 µg | 125 EUR |
Rhodamine Conjugated Affinity Purified Anti-HUMAN IgM (Fc5u) (RABBIT) |
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| GWB-97C3AD | GenWay Biotech | 1.5 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-HUMAN (lambda chain) (GOAT) |
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| GWB-B469C6 | GenWay Biotech | 1 mg | Ask for price |
Cy3 Conjugated Affinity Purified anti-Guinea Pig IgG [H&L] [Goat] |
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| MBS417874-025mg | MyBiosource | 0.25mg | 300 EUR |
Cy3 Conjugated Affinity Purified anti-Guinea Pig IgG [H&L] [Goat] |
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| SC026 | ABM | 250 µg | 145 EUR |
Biotin Conjugated Affinity Purified Anti-HUMAN IgM (mu chain) (GOAT) |
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| GWB-8DAF88 | GenWay Biotech | 1 mg | Ask for price |
Rhodamine Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (GOAT) |
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| GWB-B90DB7 | GenWay Biotech | 2 mg | Ask for price |
Rhodamine Conjugated Affinity Purified Anti-RAT IgG F(c) (RABBIT) |
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| GWB-B9AAD0 | GenWay Biotech | 1.5 mg | Ask for price |
Rhodamine Conjugated Affinity Purified Anti-MOUSE IgG F(c) (GOAT) |
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| GWB-F70082 | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-RAT IgM (mu chain) (RABBIT) |
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| GWB-CB13B0 | GenWay Biotech | 1.5 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-BOVINE IgG (H&L) (RABBIT) |
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| GWB-4F7563 | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-MOUSE IgM (mu chain) (GOAT) |
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| GWB-35EF2D | GenWay Biotech | 2 mg | Ask for price |
Rhodamine Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (GOAT) |
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| GWB-3A1563 | GenWay Biotech | 2 mg | Ask for price |
Rhodamine Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT) |
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| GWB-155325 | GenWay Biotech | 2 mg | Ask for price |
Fluorescein Conjugated Affinity Purified anti-Cat IgG [H&L] [Goat] |
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| MBS417890-025mg | MyBiosource | 0.25mg | 275 EUR |
Fluorescein Conjugated Affinity Purified anti-Dog IgG [H&L] [Goat] |
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| MBS417894-025mg | MyBiosource | 0.25mg | 275 EUR |
Fluorescein Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| MBS417914-025mg | MyBiosource | 0.25mg | 275 EUR |
Fluorescein Conjugated Affinity Purified anti-Cat IgG [H&L] [Goat] |
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| SF003 | ABM | 250 µg | 125 EUR |
Fluorescein Conjugated Affinity Purified anti-Dog IgG [H&L] [Goat] |
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| SF007 | ABM | 250 µg | 125 EUR |
Fluorescein Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat] |
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| SF027 | ABM | 250 µg | 125 EUR |
Biotin Conjugated Affinity Purified Anti-MOUSE IgG F(ab )2 (GOAT) |
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| GWB-98828F | GenWay Biotech | 2 mg | Ask for price |
Texas Red Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT) |
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| GWB-DA1826 | GenWay Biotech | 2 mg | Ask for price |
Texas Red Conjugated Affinity Purified Anti-RAT IgG F(c) (RABBIT) |
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| GWB-BAA245 | GenWay Biotech | 1.5 mg | Ask for price |
Texas Red Conjugated Affinity Purified Anti-MOUSE IgG F(c) (GOAT) |
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| GWB-BCF82F | GenWay Biotech | 2 mg | Ask for price |
Texas Red Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (GOAT) |
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| GWB-5096B8 | GenWay Biotech | 2 mg | Ask for price |
Texas Red Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (GOAT) |
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| GWB-1BEC7E | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-HUMAN IgG F(ab )2 (GOAT) |
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| GWB-879B4F | GenWay Biotech | 2 mg | Ask for price |
Texas Red Conjugated Affinity Purified anti-Cat IgG [H&L] [Rabbit] |
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| MBS417985-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Dog IgG [H&L] [Rabbit] |
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| MBS417989-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Horse IgG [H&L] [Goat] |
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| MBS417998-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Human IgG [H&L] [Goat] |
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| MBS418001-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat] |
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| MBS418004-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Rat IgG [H&L] [Donkey] |
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| MBS418008-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Swine IgG [H&L] [Goat] |
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| MBS418012-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Cat IgG [H&L] [Rabbit] |
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| ST004 | ABM | 250 µg | 125 EUR |
Texas Red Conjugated Affinity Purified anti-Dog IgG [H&L] [Rabbit] |
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| ST008 | ABM | 250 µg | 125 EUR |
Texas Red Conjugated Affinity Purified anti-Horse IgG [H&L] [Goat] |
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| ST017 | ABM | 250 µg | 125 EUR |
Texas Red Conjugated Affinity Purified anti-Human IgG [H&L] [Goat] |
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| ST020 | ABM | 250 µg | 125 EUR |
Texas Red Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat] |
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| ST023 | ABM | 250 µg | 125 EUR |
Texas Red Conjugated Affinity Purified anti-Rat IgG [H&L] [Donkey] |
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| ST027 | ABM | 250 µg | 125 EUR |
DyLight 680 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat] |
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| MBS417875-01mg | MyBiosource | 0.1mg | 350 EUR |
DyLight 649 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat] |
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| MBS417878-01mg | MyBiosource | 0.1mg | 350 EUR |
DyLight 800 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat] |
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| MBS417880-01mg | MyBiosource | 0.1mg | 350 EUR |
DyLight 488 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat] |
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| MBS417883-01mg | MyBiosource | 0.1mg | 350 EUR |
DyLight 549 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat] |
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| MBS417884-01mg | MyBiosource | 0.1mg | 350 EUR |
DyLight 680 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat] |
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| SD001 | ABM | 100 µg | 200 EUR |
DyLight 649 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat] |
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| SD004 | ABM | 100 µg | 200 EUR |
DyLight 800 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat] |
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| SD006 | ABM | 100 µg | 200 EUR |
DyLight 488 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat] |
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| SD009 | ABM | 100 µg | 200 EUR |
DyLight 549 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat] |
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| SD010 | ABM | 100 µg | 200 EUR |
Biotin Conjugated Affinity Purified Anti-CHICKEN IgG (H&L) (RABBIT) |
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| GWB-ECBD6C | GenWay Biotech | 1.5 mg | Ask for price |
Peroxidase Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (GOAT) |
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| GWB-B8E81A | GenWay Biotech | 2 mg | Ask for price |
Peroxidase Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT) |
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| GWB-F8D7A9 | GenWay Biotech | 2 mg | Ask for price |
Peroxidase Conjugated Affinity Purified Anti-MOUSE IgG F(c) (GOAT) |
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| GWB-CF3FCE | GenWay Biotech | 2 mg | Ask for price |
Rhodamine Conjugated Affinity Purified Anti-RABBIT IgG F(c) (GOAT) |
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| GWB-58D11A | GenWay Biotech | 2 mg | Ask for price |
Rhodamine Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT) |
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| GWB-5EBB84 | GenWay Biotech | 2 mg | Ask for price |
Rhodamine Conjugated Affinity Purified Anti-RABBIT IgG (H&L) (GOAT) |
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| GWB-2F3372 | GenWay Biotech | 2 mg | Ask for price |
Peroxidase Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (GOAT) |
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| GWB-30739F | GenWay Biotech | 2 mg | Ask for price |
Texas Red Conjugated Affinity Purified Anti-HUMAN IgM (Fc5u) (RABBIT) |
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| GWB-71A3CD | GenWay Biotech | 1.5 mg | Ask for price |
Rhodamine Conjugated Affinity Purified Anti-GOAT IgG F(c) (RABBIT) |
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| GWB-7A3721 | GenWay Biotech | 2 mg | Ask for price |
Peroxidase Conjugated Affinity Purified Anti-RAT IgG F(c) (RABBIT) |
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| GWB-7C1473 | GenWay Biotech | 1.5 mg | Ask for price |
Peroxidase Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat] |
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| MBS417937-1mg | MyBiosource | 1mg | 300 EUR |
Peroxidase Conjugated Affinity Purified anti-Cat IgG [H&L] [Rabbit] |
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| MBS417940-025mg | MyBiosource | 0.25mg | 275 EUR |
Peroxidase Conjugated Affinity Purified anti-Dog IgG [H&L] [Rabbit] |
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| MBS417945-025mg | MyBiosource | 0.25mg | 275 EUR |
Peroxidase Conjugated Affinity Purified anti-Horse IgG [H&L] [Goat] |
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| MBS417954-025mg | MyBiosource | 0.25mg | 275 EUR |
Peroxidase Conjugated Affinity Purified anti-Human IgG [H&L] [Goat] |
|||
| MBS417957-025mg | MyBiosource | 0.25mg | 275 EUR |
Peroxidase Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat] |
|||
| MBS417960-025mg | MyBiosource | 0.25mg | 275 EUR |
Peroxidase Conjugated Affinity Purified anti-Rat IgG [H&L] [Donkey] |
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| MBS417964-025mg | MyBiosource | 0.25mg | 275 EUR |
Peroxidase Conjugated Affinity Purified anti-Swine IgG [H&L] [Goat] |
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| MBS417968-025mg | MyBiosource | 0.25mg | 275 EUR |
Peroxidase Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat] |
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| SG023 | ABM | 1.0 mg | 145 EUR |
Peroxidase Conjugated Affinity Purified anti-Cat IgG [H&L] [Rabbit] |
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| SH003 | ABM | 250 µg | 125 EUR |
Peroxidase Conjugated Affinity Purified anti-Dog IgG [H&L] [Rabbit] |
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| SH008 | ABM | 250 µg | 125 EUR |
Peroxidase Conjugated Affinity Purified anti-Horse IgG [H&L] [Goat] |
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| SH017 | ABM | 250 µg | 125 EUR |
Peroxidase Conjugated Affinity Purified anti-Human IgG [H&L] [Goat] |
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| SH020 | ABM | 250 µg | 125 EUR |
Peroxidase Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat] |
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| SH023 | ABM | 250 µg | 125 EUR |
Peroxidase Conjugated Affinity Purified anti-Rat IgG [H&L] [Donkey] |
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| SH027 | ABM | 250 µg | 125 EUR |
Peroxidase Conjugated Affinity Purified anti-Swine IgG [H&L] [Goat] |
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| SH031 | ABM | 250 µg | 125 EUR |
Peroxidase Conjugated Affinity Purified Goat anti-Mouse IgG [H&L] |
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| TA130003 | Origene Technologies GmbH | 250 µg | Ask for price |
Rhodamine Conjugated Affinity Purified Anti-HUMAN (kappa chain) (GOAT) |
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| GWB-88E5D0 | GenWay Biotech | 1 mg | Ask for price |
Rhodamine Conjugated Affinity Purified Anti-HUMAN (lambda chain) (GOAT) |
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| GWB-8D4D52 | GenWay Biotech | 1 mg | Ask for price |
Fluorescein Conjugated Affinity Purified Anti-HUMAN IgM (Fc5u) (RABBIT) |
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| GWB-BA1E24 | GenWay Biotech | 1.5 mg | Ask for price |
Peroxidase Conjugated Affinity Purified Anti-HUMAN IgM (Fc5u) (RABBIT) |
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| GWB-561015 | GenWay Biotech | 1.5 mg | Ask for price |
Peroxidase Conjugated Affinity Purified Anti-HUMAN (kappa chain) (GOAT) |
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| GWB-26178B | GenWay Biotech | 1 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-GOAT IgG F(ab )2 (RABBIT) |
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| GWB-E4B192 | GenWay Biotech | 2 mg | Ask for price |
Texas Red Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT) |
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| GWB-F1F47D | GenWay Biotech | 2 mg | Ask for price |
Texas Red Conjugated Affinity Purified Anti-RABBIT IgG F(c) (GOAT) |
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| GWB-B991D0 | GenWay Biotech | 2 mg | Ask for price |
Biotin Conjugated Affinity Purified Anti-RABBIT IgG F(ab )2 (GOAT) |
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| GWB-B9BBFD | GenWay Biotech | 2 mg | Ask for price |
Texas Red Conjugated Affinity Purified Anti-RABBIT IgG (H&L) (GOAT) |
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| GWB-2CBA00 | GenWay Biotech | 2 mg | Ask for price |
Texas Red Conjugated Affinity Purified Anti-GOAT IgG F(c) (RABBIT) |
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| GWB-77DF57 | GenWay Biotech | 2 mg | Ask for price |
Texas Red Conjugated Affinity Purified anti-Bovine IgG [H&L] [Goat] |
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| MBS417982-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Donkey IgG [H&L] [Goat] |
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| MBS417990-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey] |
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| MBS417992-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Goat IgG [H&L] [Rabbit] |
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| MBS417993-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| MBS418007-025mg | MyBiosource | 0.25mg | 275 EUR |
Texas Red Conjugated Affinity Purified anti-Donkey IgG [H&L] [Goat] |
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| ST009 | ABM | 250 µg | 125 EUR |
Texas Red Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey] |
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| ST011 | ABM | 250 µg | 125 EUR |
Texas Red Conjugated Affinity Purified anti-Goat IgG [H&L] [Rabbit] |
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| ST012 | ABM | 250 µg | 125 EUR |
Texas Red Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| ST026 | ABM | 250 µg | 125 EUR |
DyLight 488 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| MBS417876-01mg | MyBiosource | 0.1mg | 350 EUR |
DyLight 549 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| MBS417877-01mg | MyBiosource | 0.1mg | 350 EUR |
DyLight 649 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| MBS417879-01mg | MyBiosource | 0.1mg | 350 EUR |
DyLight 680 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| MBS417881-01mg | MyBiosource | 0.1mg | 350 EUR |
DyLight 800 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
|||
| MBS417882-01mg | MyBiosource | 0.1mg | 350 EUR |
DyLight 488 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| SD002 | ABM | 100 µg | 200 EUR |
DyLight 549 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| SD003 | ABM | 100 µg | 200 EUR |
DyLight 649 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| SD005 | ABM | 100 µg | 200 EUR |
DyLight 680 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| SD007 | ABM | 100 µg | 200 EUR |
DyLight 800 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] |
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| SD008 | ABM | 100 µg | 200 EUR |
Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG F(c) (GOAT) |
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| GWB-940155 | GenWay Biotech | 2 mg | Ask for price |
Rhodamine Conjugated Affinity Purified Anti-SHEEP IgG F(c) (RABBIT) |
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| GWB-D9A0CD | GenWay Biotech | 1.5 mg | Ask for price |
Rhodamine Conjugated Affinity Purified Anti-RAT-IgG (H&L) (RABBIT) |
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| GWB-DEAE8A | GenWay Biotech | 2 mg | Ask for price |
Fluorescein Conjugated Affinity Purified Anti-RAT IgG F(c) (RABBIT) |
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| GWB-DFC7B3 | GenWay Biotech | 1.5 mg | Ask for price |
Rhodamine Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (RABBIT) |
|||
| GWB-E1B66C | GenWay Biotech | 2 mg | Ask for price |
Peroxidase Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT) |
|||
| GWB-E6CD92 | GenWay Biotech | 2 mg | Ask for price |
H-2d purificato mediante cromatografia su colonna su M1 / 42 è risultato sierologicamente e biologicamente attivo come determinato dal rebinding di MAb, dall’inibizione della lisi cellulare da parte di alloantisieri più complemento e dalla capacità di stimolare il CTL alloreattivo. Questo anticorpo e i protocolli descritti dovrebbero essere utili per la preparazione di quantità relativamente grandi di antigeni H-2 multipli.