Aprile 23, 2024

Fluorescente: proteine ​​di fusione a catena singola Fv fluorescenti verdi

Confronto del legame cellulare e dell’assorbimento di fosfodiestere, fosforotioato e oligonucleotidi antisenso misti di fosforotioato e metilfosfonato. L’effetto delle modificazioni del fosforotioato (S-oligonucleotide) o del fosfodiestere fosforotioato terminale (SO-oligonucleotidi) o del fosfodiestere metilfosfonato (MP-O-oligonucleotidi) sul legame, assorbimento e degradazione delle cellule della milza murina è stato studiato utilizzando    oligonucleotidi coniugati con fluoresceina ( FITC  )  . Gli oligonucleotidi S hanno mostrato il legame e l’assorbimento cellulare più elevati seguiti dagli oligonucleotidi SO-, O- e MP-O-.

  1. Studi sulla concorrenza hanno dimostrato che gli S-oligonucleotidi hanno una maggiore   affinità   per i siti di legame degli oligonucleotidi di membrana poiché possono bloccare completamente il legame dell’O-oligonucleotidi con un rapporto molare di appena 0,1. L’assorbimento di tutti gli oligonucleotidi era maggiore nei linfociti B rispetto ai linfociti T ed era aumentato dalla stimolazione con il mitogeno dei linfociti B, il lipopolisaccaride.
  2. Sebbene le nostre cellule siano state   purificate   utilizzando tecniche convenzionali per eliminare le cellule morte, circa il 5% delle cellule è rimasto morto o morente come determinato dalla citometria a flusso utilizzando la colorazione con ioduro di propidio. Vale la pena notare che l’associazione degli oligonucleotidi con le cellule morte era circa 50 volte maggiore rispetto alle cellule viventi.
  3. La microscopia confocale ha confermato che gli oligonucleotidi nelle cellule vitali erano intracellulari e mostravano uno scarso assorbimento nel nucleo per 4 ore. Sebbene un’estesa degradazione degli O-oligonucleotidi intracellulari fosse visibile dopo 4 ore, non vi era alcuna degradazione rilevabile di S-, SO o MP-O-oligonucleotidi.

I cumuli di cellule mesangiali. Focolai nodulari di eccessiva crescita cellulare e matrice in coltura prolungata.

  • Per studiare la capacità delle cellule mesangiali glomerulari (MC) di produrre matrice extracellulare, gli autori hanno studiato la MC in coltura utilizzando la microscopia ottica ed elettronica, nonché l’immunocitochimica. Le MC sono state ottenute da glomeruli di ratto isolati e mantenute per un massimo di 12 settimane in un mezzo contenente il 20% di siero fetale di vitello.
  • La crescita eccessiva di MC dalla coltura primaria e fino a tre sottocolture ha mostrato un’organizzazione caratteristica costituita da filamenti di cellule allungate o stellate intrecciate. Dopo la confluenza dopo 10-16 giorni, le MC hanno continuato a crescere in multistrati irregolari. Gli MC hanno prodotto materiale di matrice extracellulare entro 2-4 giorni dopo la placcatura e grandi quantità di matrice si sono accumulate nel tempo. Dopo 2-3 settimane, focolai di eccessiva proliferazione di MC, secrezione di matrice e detriti cellulari necrotici formavano sporgenze nodulari che gradualmente formavano grandi collinette.
  • Gli studi immunocitochimici di polloni MC sono stati eseguiti su piastre di coltura o su materiale asportato utilizzando specifici anticorpi policlonali di coniglio contro proteine ​​della matrice isolate e    secondi     anticorpi coniugati con FITC  e  purificati  per  affinità .
  • Durante i 3 giorni di coltivazione, le MC hanno sviluppato fibronectina e collagene di tipo I, III, IV e V. Nel tempo, i filamenti della matrice, soprattutto nella massa centrale delle colline, si sono colorati intensamente per questi componenti. La colorazione della laminina era meno pronunciata.
  • La miosina delle cellule muscolari lisce è stata regolarmente trovata su fibre intracellulari discrete e nel materiale extracellulare delle collinette. La microscopia elettronica ha mostrato che le collinette erano costituite da cellule allungate sulla superficie e cellule stellate intervallate dalla matrice e dai detriti delle cellule necrotiche nel nucleo.
  • I risultati mostrano che le MC proliferanti possono essere mantenute in colture omogenee per periodi più lunghi. Le MC producono grandi quantità di proteine ​​della matrice extracellulare (collagene di tipo IV e V, fibronectina, laminina), che si trovano nei glomeruli normali.
  • Le MC coltivate producono anche collagene interstiziale di tipo I e III. Le colline MC mostrano un accumulo di matrice nodulare simile a quello visto nel mesangio dei glomeruli malati. Si è concluso che un modello in vitro di crescita prolungata di MC potrebbe facilitare lo studio dei fattori che regolano la produzione della matrice mesangiale. Tale modello potrebbe essere utilizzato per studiare la risposta mesangiale a specifici stimoli infiammatori o metabolici.

PET / CT in vivo per i recettori EphB4 di piccoli animali utilizzando il peptide marcato con 64Cu.

  1. Molti tumori solidi sovraesprimono il recettore EphB4, un membro della famiglia del recettore dell’efrina tirosin-chinasi. L’imaging EphB4 non invasivo ha il potenziale per aumentare i tassi di diagnosi precoce, monitorare la risposta alla terapia anti-EphB4 e migliorare i risultati dei pazienti. Lo scopo di questo studio era di valutare un nuovo (64) peptide marcato con Cu con un’elevata   affinità  per il legame dei recettori PET ai recettori EphB4.
  2. TNYLFSPNGPIARAW Il peptide legante EphB4 (TNYL-RAW) è stato coniugato con isotiocianato di fluoresceina (FITC) e DOTA. DOTA-TNYL-RAW è stato etichettato con (64) Cu con un’elevata efficienza di etichettatura. L’ affinità di legame  di TNYL-RAW e dei suoi derivati ​​per EphB4 ricombinante purificato è stata determinata utilizzando la tecnologia di risonanza plasmonica di superficie. Il legame in vitro di FITC-TNYL-RAW e (64) Cu-DOTA-TNYL-RAW alle cellule tumorali è stato valutato mediante microscopia a fluorescenza e metodi di conteggio della radioattività. La biodistribuzione in vivo e la PET/TC in piccoli animali sono state eseguite in topi portatori di tumori CT26 e PC-3M che esprimono EphB4, nonché di tumori A549 negativi per EphB4.
  3. TNYL-RAW e suoi derivati ​​hanno mostrato un’elevata   affinità di legame a EphB4, con una costante di equilibrio di dissociazione di 1,98-23 nM. In vitro, sia FITC-TNYL-RAW che (64) Cu-DOTA-TNYL-RAW sono stati assorbiti selettivamente dalle cellule CT26 e PC-3M, ma non dalle cellule A549. Il legame di FITC-TNYL-RAW e (64) Cu-DOTA-TNYL-RAW alle cellule CT26 e PC-3M può essere bloccato dall’eccesso di TNYL-RAW. In vivo, (64) Cu-DOTA-TNYL-RAW ha mostrato un assorbimento significativamente maggiore nei tumori PC-3M rispetto ai tumori A549, con una dose percentuale iniettata per grammo di tumore pari a 0,84 ± 0,09 e 0,44 ± 0,09 24 ore dopo l’iniezione del radiotracciante , rispettivamente. La PET / CT in piccoli animali ha rivelato chiaramente (64) la deposizione di Cu-DOTA-TNYL-RAW nei tumori CT26 e PC-3M, ma non nei tumori A549. Inoltre, l’assorbimento (64) di Cu-DOTA-TNYL-RAW nei tumori CT26 e PC-3M può essere bloccato dal TNYL-RAW freddo.
  • È stato progettato un sistema di espressione (pSKGFP) che consente l’espressione di frammenti variabili a catena singola come proteine ​​di fusione con proteine ​​fluorescenti verdi modificate. Questo sistema di espressione è paragonabile ai vettori di visualizzazione dei fagi comunemente usati e consente una caratterizzazione in un unico passaggio di anticorpi ricombinanti selezionati mediante citometria a flusso o colorazione cellulare fluorescente.
  • Due diversi anticorpi a frammento variabile a catena singola, entrambi diretti contro Ralstonia solanacearum lipopolisaccaridi, sono stati geneticamente fusi con una proteina fluorescente verde spostata in rosso e la proteina di fusione risultante è stata testata per l’utilità.
  • Questi fluocorpi possono essere prodotti in colture cellulari batteriche e  purificati mediante  cromatografia  per affinità con   metalli immobilizzati . Funzionano bene nella citometria a flusso e nella colorazione immunofluorescente delle cellule, sono specifici per i loro antigeni bersaglio e, a differenza  degli anticorpi coniugati  con FITC  , non sbiadiscono quando irradiati.

Caratterizzazione dell’anticorpo monoclonale anti-H-2 e suo utilizzo nella purificazione dell’antigene su larga scala.

  1. È stato riscontrato che un anticorpo monoclonale (MAb) di ratto anti-topo, M1/42, reagisce con gli antigeni H-2 degli aplotipi a, b, d, j, k, siu (tutti gli aplotipi testati). Questo anticorpo, dopo essersi legato alle cellule e aver reagito con   FITC  –    Ig anti-ratto coniugato , può essere utilizzato per quantificare l’espressione di H-2 su diversi tipi cellulari.
  2. L’anticorpo è stato anche utile per confrontare i prodotti H-2 precipitati da diversi aplotipi. Perline di sefarosio-4B accoppiate a M1/42 sono state utilizzate per purificare gli antigeni H-2d mediante cromatografia  di affinità .
  3. Molecole di H-2 pure sono state eluite dalla colonna in DOC allo 0,5%, NaCl 0,65 M, Tris 20 mM, pH 8,0, producendo da 110 a 180 microgrammi di cellule tumorali H-2d / 10 (10) P815. Questo anticorpo, utilizzato in serie con MAb specifici per H-2Kk 11-4.1, ha consentito la purificazione di Dk e Dd rispettivamente dalle cellule RDM-4 e YAC.

Cy5 Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey]

SC008 ABM 250 µg 145 EUR

Cy2 Conjugated Affinity Purified anti-Mouse IgG [H&L] [Donkey]

SC011 ABM 250 µg 145 EUR

Cy3 Conjugated Affinity Purified anti-Hamster IgG [H&L] [Goat]

SC019 ABM 250 µg 145 EUR

Cy3 Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey]

SC020 ABM 250 µg 145 EUR

Biotin Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT)

GWB-460460 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (GOAT)

GWB-4AAFE1 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-MOUSE IgG F(c) (GOAT)

GWB-175D9B GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-RAT IgG F(c) (RABBIT)

GWB-B4EC30 GenWay Biotech 1.5 mg Ask for price

Biotin Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (GOAT)

GWB-327A5B GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-HUMAN IgM (Fc5u) (RABBIT)

GWB-B1AFAF GenWay Biotech 1.5 mg Ask for price

Biotin Conjugated Affinity Purified Anti-GOAT IgG F(c) (RABBIT)

GWB-50BF10 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-RABBIT IgG (H&L) (GOAT)

GWB-16F495 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT)

GWB-E14E58 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-RABBIT IgG F(c) (GOAT)

GWB-FEFD15 GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified anti-Cat IgG [H&L] [Goat]

ST003 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Dog IgG [H&L] [Goat]

ST007 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

ST028 ABM 250 µg 125 EUR

DyLight 488 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

SD011 ABM 100 µg 200 EUR

DyLight 549 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

SD012 ABM 100 µg 200 EUR

Biotin Conjugated Affinity Purified Anti-HUMAN (kappa chain) (GOAT)

GWB-77D4DD GenWay Biotech 1 mg Ask for price

Biotin Conjugated Affinity Purified Anti-HUMAN IgG F(c) (RABBIT)

GWB-5D1187 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (RABBIT)

GWB-4515A4 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-SHEEP IgG (H&L) (RABBIT)

GWB-1C237F GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-SHEEP IgG F(c) (RABBIT)

GWB-A03546 GenWay Biotech 1.5 mg Ask for price

Biotin Conjugated Affinity Purified anti-Mouse IgG F(c) [Rabbit]

GWB-D9ED60 GenWay Biotech 1.5 mg Ask for price

Biotin Conjugated Affinity Purified anti-Mouse IgG [H&L] [Rabbit]

GWB-EAA016 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-RAT-IgG (H&L) (RABBIT)

GWB-832C22 GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified anti-Cat IgG [H&L] [Goat]

SH004 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Dog IgG [H&L] [Goat]

SH007 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

SH028 ABM 250 µg 125 EUR

Cy3 Conjugated Affinity Purified anti-Guinea Pig IgG [H&L] [Goat]

SC026 ABM 250 µg 145 EUR

Biotin Conjugated Affinity Purified Anti-HUMAN (lambda chain) (GOAT)

GWB-B469C6 GenWay Biotech 1 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-HUMAN IgM (Fc5u) (RABBIT)

GWB-97C3AD GenWay Biotech 1.5 mg Ask for price

Biotin Conjugated Affinity Purified Anti-BOVINE IgG (H&L) (RABBIT)

GWB-4F7563 GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT)

GWB-155325 GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (GOAT)

GWB-3A1563 GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (GOAT)

GWB-B90DB7 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-MOUSE IgM (mu chain) (GOAT)

GWB-35EF2D GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-HUMAN IgM (mu chain) (GOAT)

GWB-8DAF88 GenWay Biotech 1 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-MOUSE IgG F(c) (GOAT)

GWB-F70082 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-RAT IgM (mu chain) (RABBIT)

GWB-CB13B0 GenWay Biotech 1.5 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-RAT IgG F(c) (RABBIT)

GWB-B9AAD0 GenWay Biotech 1.5 mg Ask for price

Fluorescein Conjugated Affinity Purified anti-Cat IgG [H&L] [Goat]

SF003 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Dog IgG [H&L] [Goat]

SF007 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

SF027 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (GOAT)

GWB-5096B8 GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (GOAT)

GWB-1BEC7E GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-MOUSE IgG F(ab )2 (GOAT)

GWB-98828F GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified anti-Cat IgG [H&L] [Rabbit]

ST004 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Dog IgG [H&L] [Rabbit]

ST008 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Horse IgG [H&L] [Goat]

ST017 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Human IgG [H&L] [Goat]

ST020 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat]

ST023 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Rat IgG [H&L] [Donkey]

ST027 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT)

GWB-DA1826 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-HUMAN IgG F(ab )2 (GOAT)

GWB-879B4F GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-RAT IgG F(c) (RABBIT)

GWB-BAA245 GenWay Biotech 1.5 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-MOUSE IgG F(c) (GOAT)

GWB-BCF82F GenWay Biotech 2 mg Ask for price

DyLight 680 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat]

SD001 ABM 100 µg 200 EUR

DyLight 649 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat]

SD004 ABM 100 µg 200 EUR

DyLight 800 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat]

SD006 ABM 100 µg 200 EUR

DyLight 488 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat]

SD009 ABM 100 µg 200 EUR

DyLight 549 Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat]

SD010 ABM 100 µg 200 EUR

Rhodamine Conjugated Affinity Purified Anti-RABBIT IgG (H&L) (GOAT)

GWB-2F3372 GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (GOAT)

GWB-30739F GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-RABBIT IgG F(c) (GOAT)

GWB-58D11A GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (GOAT)

GWB-B8E81A GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT)

GWB-5EBB84 GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-GOAT IgG F(c) (RABBIT)

GWB-7A3721 GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-RAT IgG F(c) (RABBIT)

GWB-7C1473 GenWay Biotech 1.5 mg Ask for price

Peroxidase Conjugated Affinity Purified Goat anti-Mouse IgG [H&L]

TA130003 Origene Technologies GmbH 250 µg Ask for price

Biotin Conjugated Affinity Purified Anti-CHICKEN IgG (H&L) (RABBIT)

GWB-ECBD6C GenWay Biotech 1.5 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-HUMAN IgM (Fc5u) (RABBIT)

GWB-71A3CD GenWay Biotech 1.5 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT)

GWB-F8D7A9 GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-MOUSE IgG F(c) (GOAT)

GWB-CF3FCE GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified anti-Mouse IgG (H&L) [Goat]

SG023 ABM 1.0 mg 145 EUR

Peroxidase Conjugated Affinity Purified anti-Cat IgG [H&L] [Rabbit]

SH003 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Dog IgG [H&L] [Rabbit]

SH008 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Horse IgG [H&L] [Goat]

SH017 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Human IgG [H&L] [Goat]

SH020 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat]

SH023 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Rat IgG [H&L] [Donkey]

SH027 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Swine IgG [H&L] [Goat]

SH031 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified Anti-RABBIT IgG (H&L) (GOAT)

GWB-2CBA00 GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified anti-Donkey IgG [H&L] [Goat]

ST009 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey]

ST011 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Goat IgG [H&L] [Rabbit]

ST012 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]

ST026 ABM 250 µg 125 EUR

Biotin Conjugated Affinity Purified Anti-GOAT IgG F(ab )2 (RABBIT)

GWB-E4B192 GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-GOAT IgG F(c) (RABBIT)

GWB-77DF57 GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT)

GWB-F1F47D GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-RABBIT IgG F(c) (GOAT)

GWB-B991D0 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-RABBIT IgG F(ab )2 (GOAT)

GWB-B9BBFD GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-HUMAN (kappa chain) (GOAT)

GWB-26178B GenWay Biotech 1 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-HUMAN IgM (Fc5u) (RABBIT)

GWB-561015 GenWay Biotech 1.5 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-HUMAN (kappa chain) (GOAT)

GWB-88E5D0 GenWay Biotech 1 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-HUMAN (lambda chain) (GOAT)

GWB-8D4D52 GenWay Biotech 1 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-HUMAN IgM (Fc5u) (RABBIT)

GWB-BA1E24 GenWay Biotech 1.5 mg Ask for price

DyLight 488 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]

SD002 ABM 100 µg 200 EUR

DyLight 549 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]

SD003 ABM 100 µg 200 EUR

DyLight 649 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]

SD005 ABM 100 µg 200 EUR

DyLight 680 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]

SD007 ABM 100 µg 200 EUR

DyLight 800 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]

SD008 ABM 100 µg 200 EUR

Fluorescein Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (GOAT)

GWB-1F6A54 GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-MOUSE IgG F(c) (RABBIT)

GWB-250956 GenWay Biotech 1.5 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-MOUSE IgG F(c) (GOAT)

GWB-1934F3 GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-HUMAN IgG F(c) (RABBIT)

GWB-AC2261 GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG (H&L) (GOAT)

GWB-B3DFEC GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-SHEEP IgG (H&L) (RABBIT)

GWB-5F5155 GenWay Biotech 2 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-HUMAN IgG F(c) (GOAT)

GWB-7BA0FB GenWay Biotech 2 mg Ask for price

Phycoerythrin Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

SP005 ABM 250 µg 255 EUR

Rhodamine Conjugated Affinity Purified Anti-SHEEP IgG F(c) (RABBIT)

GWB-D9A0CD GenWay Biotech 1.5 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-RAT-IgG (H&L) (RABBIT)

GWB-DEAE8A GenWay Biotech 2 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-RAT IgG F(c) (RABBIT)

GWB-DFC7B3 GenWay Biotech 1.5 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (RABBIT)

GWB-E1B66C GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT)

GWB-E6CD92 GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG F(c) (GOAT)

GWB-940155 GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (RABBIT)

GWB-6D56DA GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-HUMAN (kappa chain) (GOAT)

GWB-F3CD76 GenWay Biotech 1 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (GOAT)

GWB-F7A9D0 GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-GOAT IgG F(c) (RABBIT)

GWB-BFEE60 GenWay Biotech 2 mg Ask for price

Fluorescein Conjugated Affinity Purified anti-Cat IgG [H&L] [Rabbit]

SF004 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Dog IgG [H&L] [Rabbit]

SF008 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Horse IgG [H&L] [Goat]

SF017 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Human IgG [H&L] [Goat]

SF020 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat]

SF023 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Rat IgG [H&L] [Donkey]

SF028 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Swine IgG [H&L][Goat]

SF031 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Bovine IgG [H&L] [Goat]

SH001 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Donkey IgG [H&L] [Goat]

SH009 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey]

SH011 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Goat IgG [H&L] [Rabbit]

SH012 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]

SH026 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (RABBIT)

GWB-202F3D GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-SHEEP IgG F(c) (RABBIT)

GWB-20AA98 GenWay Biotech 1.5 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-SHEEP IgG (H&L) (RABBIT)

GWB-25940D GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-SHEEP IgG F(ab )2 (RABBIT)

GWB-157473 GenWay Biotech 1.5 mg Ask for price

Biotin Conjugated Affinity Purified Anti-HUMAN IgG F(ab )2 (RABBIT)

GWB-B61E07 GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-HUMAN IgG F(c) (RABBIT)

GWB-980DBF GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-MOUSE IgG F(c) (RABBIT)

GWB-30FB75 GenWay Biotech 1.5 mg Ask for price

Texas Red Conjugated Affinity Purified anti-Chicken IgG [H&L] [Goat]

ST005 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Hamster IgG [H&L] [Goat]

ST015 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Horse IgG [H&L] [Rabbit]

ST018 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey]

ST019 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Human IgG [H&L] [Rabbit]

ST021 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Mouse IgG [H&L] [Donkey]

ST022 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Mouse IgG [H&L] [Rabbit]

ST024 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Sheep IgG [H&L] [Donkey]

ST029 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Sheep IgG [H&L] [Rabbit]

ST030 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (RABBIT)

GWB-E88447 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified anti-Mouse IgG F(ab’)2 [Rabbit]

GWB-84D8FC GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-RAT-IgG (H&L) (RABBIT)

GWB-F49916 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-HUMAN IgA (alpha chain) (GOAT)

GWB-266725 GenWay Biotech 1 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (Rabbit)

GWB-5998AE GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-HUMAN IgG F(c) (RABBIT)

GWB-127DEC GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-BOVINE IgG (H&L) (RABBIT)

GWB-39A1E4 GenWay Biotech 2 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-GOAT IgG F(c) (RABBIT)

GWB-463E53 GenWay Biotech 2 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (RABBIT)

GWB-4A7B1C GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-RAT-IgG (H&L) (RABBIT)

GWB-15E964 GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-HUMAN IgM (mu chain) (GOAT)

GWB-1660C7 GenWay Biotech 1 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-HUMAN IgG F(c) (RABBIT)

GWB-A2A5E7 GenWay Biotech 2 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (RABBIT)

GWB-A1B7F7 GenWay Biotech 2 mg Ask for price

Biotin Conjugated Affinity Purified Anti-HUMAN IgG (gamma chain) (GOAT)

GWB-7DCC82 GenWay Biotech 1 mg Ask for price

Phycoerythrin Conjugated Affinity Purified anti-Human IgG [H&L] [Goat]

SP002 ABM 250 µg 255 EUR

Phycoerythrin Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat]

SP003 ABM 250 µg 255 EUR

Fluorescein Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT)

GWB-D9E4DC GenWay Biotech 2 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-RAT-IgG (H&L) (RABBIT)

GWB-DCC1C2 GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG (H&L) (RABBIT)

GWB-E6B610 GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-HUMAN (lambda chain) (GOAT)

GWB-EB2FA4 GenWay Biotech 1 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-RABBIT IgG (H&L) (GOAT)

GWB-81642B GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-MOUSE IgM (mu chain) (GOAT)

GWB-84AC02 GenWay Biotech 2 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-RABBIT IgG F(c) (GOAT)

GWB-6BD4A4 GenWay Biotech 2 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-SHEEP IgG (H&L) (RABBIT)

GWB-6C7711 GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-MOUSE IgM (mu chain) (GOAT)

GWB-760157 GenWay Biotech 1 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-HUMAN IgG (H&L) (RABBIT)

GWB-F60FF1 GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-CHICKEN IgG (H&L) (RABBIT)

GWB-F853EB GenWay Biotech 1.5 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-SHEEP IgG F(c) (RABBIT)

GWB-F9560D GenWay Biotech 1.5 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-RAT IgM (mu chain) (RABBIT)

GWB-FAEBBD GenWay Biotech 1.5 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (RABBIT)

GWB-FBC089 GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(c) (RABBIT)

GWB-C753EB GenWay Biotech 1.5 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-HUMAN IgM (mu chain) (GOAT)

GWB-CC87A1 GenWay Biotech 1 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-RAT IgM (mu chain) (RABBIT)

GWB-BD9D77 GenWay Biotech 1.5 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-MOUSE IgG F(c) (RABBIT)

GWB-C11791 GenWay Biotech 1.5 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-BOVINE IgG (H&L) (RABBIT)

GWB-C158C0 GenWay Biotech 2 mg Ask for price

Fluorescein Conjugated Affinity Purified Anti-MOUSE IgG F(c) (RABBIT)

GWB-C512D5 GenWay Biotech 1.5 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-MOUSE (kappa chain) (RABBIT)

GWB-D64E40 GenWay Biotech 1 mg Ask for price

Fluorescein Conjugated Affinity Purified anti-Chicken IgG [H&L] [Goat]

SF005 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Donkey IgG [H&L] [Goat]

SF009 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey]

SF011 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Goat IgG [H&L] [Rabbit]

SF012 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Hamster IgG [H&L] [Goat]

SF015 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Horse IgG [H&L] [Rabbit]

SF018 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey]

SF019 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Human IgG (H&L) [Rabbit]

SF021 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Mouse IgG [H&L] [Donkey]

SF022 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Mouse IgG [H&L] [Rabbit]

SF024 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]

SF026 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Sheep IgG [H&L] [Donkey]

SF029 ABM 250 µg 125 EUR

Fluorescein Conjugated Affinity Purified anti-Sheep IgG [H&L] [Rabbit]

SF030 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Bovine IgG [H&L] [Rabbit]

SH002 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Chicken IgG [H&L] [Goat]

SH005 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Donkey IgG [H&L] [Rabbit]

SH010 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Horse IgG [H&L] [Rabbit]

SH018 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey]

SH019 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Human IgG [H&L] [Rabbit]

SH021 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Mouse IgG [H&L] [Donkey]

SH022 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Mouse IgG [H&L] [Rabbit]

SH024 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Donkey]

SH025 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Sheep IgG [H&L] [Donkey]

SH029 ABM 250 µg 125 EUR

Peroxidase Conjugated Affinity Purified anti-Sheep IgG [H&L] [Rabbit]

SH030 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified Anti-HUMAN IgG F(ab )2 (GOAT)

GWB-DA295E GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-MOUSE IgG F(ab )2 (GOAT)

GWB-70D539 GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-RAT IgG F(ab )2 (RABBIT)

GWB-CA89F0 GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-MOUSE IgG F(ab )2 (GOAT)

GWB-45BC14 GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-HUMAN IgM (mu chain) (GOAT)

GWB-AA76A1 GenWay Biotech 1 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-RAT IgM (mu chain) (RABBIT)

GWB-B94BFA GenWay Biotech 1.5 mg Ask for price

Texas Red Conjugated Affinity Purified anti-Bovine IgG [H&L] [Rabbit]

ST002 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Donkey IgG [H&L] [Rabbit]

ST010 ABM 250 µg 125 EUR

Texas Red Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Donkey]

ST025 ABM 250 µg 125 EUR

Rhodamine Conjugated Affinity Purified Anti-RAT IgG F(ab )2 (RABBIT)

GWB-E7B130 GenWay Biotech 2 mg Ask for price

Rhodamine Conjugated Affinity Purified Anti-HUMAN IgG F(ab )2 (GOAT)

GWB-F21370 GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-MOUSE IgM (mu chain) (GOAT)

GWB-FE8F97 GenWay Biotech 2 mg Ask for price

Texas Red Conjugated Affinity Purified Anti-BOVINE IgG (H&L) (RABBIT)

GWB-C6EC10 GenWay Biotech 2 mg Ask for price

Peroxidase Conjugated Affinity Purified Anti-HUMAN (lambda chain) (GOAT)

GWB-5A8F52 GenWay Biotech 1 mg Ask for price

H-2d   purificato   mediante cromatografia su colonna su M1 / ​​42 è risultato sierologicamente e biologicamente attivo come determinato dal rebinding di MAb, dall’inibizione della lisi cellulare da parte di alloantisieri più complemento e dalla capacità di stimolare il CTL alloreattivo. Questo anticorpo e i protocolli descritti dovrebbero essere utili per la preparazione di quantità relativamente grandi di antigeni H-2 multipli.

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