Ottobre 13, 2026
Biology Cells / cDNA / Clia Kits / Devices / Gels / Isotypes / Particles

ELISA kits for the screening of colistin residue in porcine and poultry muscle based on the European guideline

Andreaby Andrea

Characterization of Aggressive ELISA and Formulated Alhydrogel Aggressive ELISA (FAcE) for Direct Quantification of Energetic Substances in GMMA-Primarily based Vaccines

Generalized modules for membrane antigens (GMMA) characterize a know-how significantly engaging for designing reasonably priced vaccines in opposition to Gram-negative micro organism. We explored such know-how for the event of O-antigen-based vaccines in opposition to Shigella and nontyphoidal Salmonella. Adsorption of GMMA on Alhydrogel was required for abrogation of pyrogenicity in rabbits, and Shigella sonnei GMMA on Alhydrogel was nicely tolerated and immunogenic in people.

Quantification of key antigens in formulated vaccines was elementary for launch and to examine stability additional time. Historically, the direct quantification of antigens adsorbed on aluminum salts has been difficult, and the quantification of every energetic ingredient in multicomponent formulated vaccines has been much more sophisticated. To immediately quantify every energetic ingredient and unbound drug substances in formulated vaccines, we developed the Formulated Alhydrogel aggressive ELISA (FAcE) and the aggressive ELISA technique, respectively. 

The strategies had been each absolutely characterised, assessing specificity, repeatability, intermediate precision, and accuracy, for S. sonnei OAg quantification, each in a single part or multicomponent GMMA formulation additionally containing S. flexneri GMMA. The developed immunological strategies allowed us to completely characterize Shigella GMMA drug merchandise, supporting their preclinical and medical improvement. The identical strategies, already prolonged to GMMA from nontyphoidal Salmonella and Neisseria meningitidis, might be doubtlessly prolonged to any antigen formulated on Alhydrogel. 

Analysis of three ELISA kits for the screening of colistin residue in porcine and poultry muscle based on the European guideline for the validation of screening strategies

Colistin is a polypeptide antibiotic primarily utilized in porcine and poultry to deal with gastrointestinal infections. It has been included by the World Well being Organisation (WHO) within the listing of critically essential human antibiotics of excessive precedence for antimicrobial resistance since 2017. Due to this fact, it’s essential to develop particular and delicate screening strategies for this molecule. Screening for colistin with immunoassays is an fascinating different to LC-MS/MS screening strategies.

The efficiency of three commercially obtainable ELISA kits was evaluated in poultry and porcine muscular tissues for the detection of colistin with reference to its European most residue restrict (MRL) (150 µg/kg). The applicability of the three ELISA kits to the detection of colistin at or beneath the MRL in porcine and poultry muscular tissues was demonstrated. The detection capabilities (CCβ) of two kits had been or decrease than or equal to the MRL (150 µg/kg). The bottom detection functionality (30 µg/kg) was achieved with the third ELISA package. The specificity of the three kits was very passable (false optimistic charges 0%). The three kits are very particular for the detection of colistin (colistin A and B) and polymyxin B.   

Canine Creatinine ELISA kit
E01A63413 96T
EUR 700
Description: ELISA

Canine Creatinine ELISA kit
E08C0629-48wellsplate 48 wells plate
EUR 280

Canine Creatinine ELISA kit
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Canine Creatinine ELISA Kit
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EUR 5685

Canine Creatinine ELISA Kit
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Canine Creatinine (Cre) ELISA Kit
MBS2611104-10x96StripWells 10x96-Strip-Wells
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Canine Creatinine (Cre) ELISA Kit
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Canine Creatinine (Cre) ELISA Kit
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Canine Creatinine (Cre) ELISA Kit
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Canine creatinine(Cre) ELISA Kit
NSL1403Ca 1498 wells
EUR 528
Description: Sandwich ELISA

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abx350661-96tests 96 tests
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Creatinine (CR) ELISA Kit
abx257719-10g 10 µg
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Creatinine (CR) ELISA Kit
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Creatinine (CR) ELISA Kit
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Creatinine (Cr) ELISA Kit
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Cr (creatinine) ELISA Kit
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Cr (creatinine) ELISA Kit
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Cr (creatinine) ELISA Kit
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Cat Creatinine,Cr ELISA KIT
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Cat Creatinine,Cr ELISA KIT
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Cat Creatinine,Cr ELISA KIT
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EUR 2137

Cat Creatinine,Cr ELISA KIT
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Rat Creatinine (Cr) ELISA Kit
AE29960RA-48Tests 48 Tests
EUR 325
Description: Rat (Rattus norvegicus)

Rat Creatinine (Cr) ELISA Kit
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EUR 610
Description: Rat (Rattus norvegicus)

Rat Creatinine(Cr)ELISA Kit
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EUR 380.4

Rat Creatinine(Cr)ELISA Kit
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EUR 595.2

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EUR 366.4
Description: Rat

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EUR 628.92
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Rattus;Sensitivity: 0.75nmol/ml

Rat Creatinine, Cr ELISA Kit
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EUR 3955

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Rat Creatinine, Cr ELISA Kit
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SL0204Ra -
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YLA0560RA-48T 48T Ask for price

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201-12-1090 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Mouse Creatinine (Cr) ELISA Kit
AE58163MO-48T 48T
EUR 325
Description: Mouse (Mus musculus)

Mouse Creatinine (Cr) ELISA Kit
AE58163MO-48Tests 48 Tests
EUR 325
Description: Mouse (Mus musculus)

Mouse Creatinine (Cr) ELISA Kit
AE58163MO-96T 96T
EUR 610
Description: Mouse (Mus musculus)

Mouse Creatinine (Cr) ELISA Kit
AE58163MO-96Tests 96 Tests
EUR 610
Description: Mouse (Mus musculus)

Human Creatinine (Cr) ELISA Kit
AE59008HU-48T 48T
EUR 360
Description: Human (Homo sapiens)

Human Creatinine (Cr) ELISA Kit
AE59008HU-48Tests 48 Tests
EUR 360
Description: Human (Homo sapiens)

Human Creatinine (Cr) ELISA Kit
AE59008HU-96T 96T
EUR 680
Description: Human (Homo sapiens)

Human Creatinine (Cr) ELISA Kit
AE59008HU-96Tests 96 Tests
EUR 680
Description: Human (Homo sapiens)

Mouse Cr(Creatinine) ELISA Kit
EM0950 96T
EUR 628.92
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Mus ;Sensitivity: 0.75nmol/ml

Human Creatinine(Cr)ELISA Kit
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EUR 346.8

Human Creatinine(Cr)ELISA Kit
GA-E1106HM-48Tests 48 Tests Ask for price

Human Creatinine(Cr)ELISA Kit
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EUR 559.2

Human Creatinine(Cr)ELISA Kit
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Human Creatinine,CR ELISA KIT
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EUR 366.4
Description: Human

Human Creatinine (Cr) ELISA Kit
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EUR 799

Mouse Creatinine (Cr) ELISA Kit
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Human Creatinine,CR ELISA KIT
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Human Creatinine(Cr) Elisa Kit
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EUR 0.12

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Human Cr(Creatinine) ELISA Kit
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EUR 571.5
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 0.75nmol/ml

Human Creatinine (Cr) ELISA Kit
KTE62273-48T 48T
EUR 359
Description: Human Creatinine (Cr) ELISA Kit employs a two-site sandwich ELISA to quantitate Creatinine in samples.

Human Creatinine (Cr) ELISA Kit
KTE62273-96T 96T
EUR 619
Description: Human Creatinine (Cr) ELISA Kit employs a two-site sandwich ELISA to quantitate Creatinine in samples.

Human Creatinine (Cr) ELISA Kit
KTE62273-96T5 96 T×5
EUR 2809
Description: Human Creatinine (Cr) ELISA Kit employs a two-site sandwich ELISA to quantitate Creatinine in samples.

Human Creatinine (Cr) ELISA Kit
KTE62273-96T50 96 T×50
EUR 26209
Description: Human Creatinine (Cr) ELISA Kit employs a two-site sandwich ELISA to quantitate Creatinine in samples.

Human Creatinine, CR ELISA Kit
MBS1609962-10x96StripWells 10x96-Strip-Wells
EUR 3460

Human Creatinine, CR ELISA Kit
MBS1609962-5x96StripWells 5x96-Strip-Wells
EUR 1750

Human Creatinine, CR ELISA Kit
MBS1609962-96StripWells 96-Strip-Wells
EUR 425

Mouse Creatinine (CR) ELISA Kit
MBS3805479-10x96StripWells 10x96-Strip-Wells
EUR 6725

Mouse Creatinine (CR) ELISA Kit
MBS3805479-48StripWells 48-Strip-Wells
EUR 550

Mouse Creatinine (CR) ELISA Kit
MBS3805479-5x96StripWells 5x96-Strip-Wells
EUR 3420

Mouse Creatinine (CR) ELISA Kit
MBS3805479-96StripWells 96-Strip-Wells
EUR 765

Mouse Cr (Creatinine) ELISA Kit
MBS2504918-10x96StripWells 10x96-Strip-Wells
EUR 3925

Mouse Cr (Creatinine) ELISA Kit
MBS2504918-24StripWells 24-Strip-Wells
EUR 225

Mouse Cr (Creatinine) ELISA Kit
MBS2504918-48StripWells 48-Strip-Wells
EUR 455

Mouse Cr (Creatinine) ELISA Kit
MBS2504918-5x96StripWells 5x96-Strip-Wells
EUR 2205

Mouse Cr (Creatinine) ELISA Kit
MBS2504918-96StripWells 96-Strip-Wells
EUR 540

Human Creatinine (Cr) ELISA Kit
MBS287752-10x96StripWells 10x96-Strip-Wells
EUR 6840

Human Creatinine (Cr) ELISA Kit
MBS287752-48StripWells 48-Strip-Wells
EUR 480

Human Creatinine (Cr) ELISA Kit
MBS287752-5x96StripWells 5x96-Strip-Wells
EUR 3610

Human Creatinine (Cr) ELISA Kit
MBS287752-96StripWells 96-Strip-Wells
EUR 805

Mouse Creatinine (CR) ELISA Kit
MBS285849-10x96StripWells 10x96-Strip-Wells
EUR 6255

Mouse Creatinine (CR) ELISA Kit
MBS285849-48StripWells 48-Strip-Wells
EUR 445

Mouse Creatinine (CR) ELISA Kit
MBS285849-5x96StripWells 5x96-Strip-Wells
EUR 3290

Mouse Creatinine (CR) ELISA Kit
MBS285849-96StripWells 96-Strip-Wells
EUR 730

Mouse Cr (Creatinine) ELISA Kit
MBS7612370-10x96StripWells 10x96-Strip-Wells
EUR 3900

Detection of Locked Nucleic Acid Gapmers from Mouse Muscle Samples Utilizing ELISA

Antisense oligonucleotide (ASO)-mediated remedy is promising for the remedy of quite a lot of genetic problems, akin to Duchenne muscular dystrophy. As extra ASOs advance in therapeutic improvement and enter medical trials, it turns into essential to have a method of quantifying their quantities in organic samples post-treatment. This data can be useful for evaluating the protection and pharmacokinetic profiles of ASOs, and in deciding how the efficacy of those medicine could be improved.

Gapmers are a category of ASOs characterised by having a central DNA portion that’s surrounded by chemically modified nucleotides on each ends. Whereas comparatively easy and accessible strategies to quantify different ASOs akin to phosphorodiamidate morpholino oligomers (PMOs) utilizing enzyme-linked immunosorbent assay (ELISA)-based methods can be found and have been used for in vivo research, no such technique is accessible for gapmers to our data. Right here, we describe a delicate ELISA protocol that can be utilized to quantify the degrees of locked nucleic acid (LNA) gapmers in mouse muscle tissue. 

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