Gennaio 24, 2025

Gene Copy Quantity Variation Genotyping by Excessive Decision Melting PCR

Response to “No proof of SARS-CoV-2 an infection by PCR or serology in kids with pseudochilblain”

We learn with curiosity the article by Caselli D et al., reporting a case collection of 38 kids with chilblain-like lesions (CLL). Testing for SARS-CoV-2, together with PCR, fast take a look at serology and ELISA methodology for IgA and IgG antibodies yielded adverse ends in all instances. They concluded that their knowledge don’t enable them to assist the connection of CLL with SARS-CoV-2 an infection. To date, knowledge within the literature learning CLL documented a really low share of laboratory confirmed SARS-CoV-2. Nevertheless, Colmenero and colleagues have been in a position to detect SARS-CoV-2 in endothelial cells of cutaneous chilblain lesions by immunohistochemistry strategies in 7 pediatric sufferers with adverse nasopharyngeal swabs.

Complement C4 Gene Copy Quantity Variation Genotyping by Excessive Decision Melting PCR

Background: Complement C4 gene copy quantity variation performs an essential position as a determinant of genetic susceptibility to frequent illnesses, similar to systemic lupus erythematosus, schizophrenia, rheumatoid arthritis, and infectious illnesses. This research aimed to develop an assay for the quantification of copy quantity variations within the C4 locus.

Strategies: the assay was based mostly on a gene ratio evaluation copy enumeration (GRACE) PCR mixed with excessive decision melting (HRM) PCR. The take a look at was optimized utilizing samples of a recognized genotype and validated with 72 DNA samples from wholesome blood donors.

Outcomes: to validate the assay, customary curves have been generated by plotting the C4/RP1 ratio values towards copy quantity variation (CNV) for every gene, utilizing genomic DNA with recognized C4 CNV. The vary of copy numbers in management people was similar to distributions noticed in earlier research of European descent.

Conclusions: the tactic herein described considerably simplifies C4 CNV analysis to validate the assay.

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Description: Orphan GPCR SP9155 agonist P550 (mouse, rat)

Orphan GPCR SP9155 agonist P550 (mouse, rat)

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Description: Orphan GPCR SP9155 agonist P550 (mouse, rat)

Orphan GPCR SP9155 agonist P550 (mouse, rat)

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Description: Orphan GPCR SP9155 agonist P550 (mouse, rat)

Orphan GPCR SP9155 agonist P550 (mouse, rat)

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Description: Orphan GPCR SP9155 agonist P550 (mouse, rat)

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

MBS5785731-10mg 10(mg
EUR 645

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

MBS5785731-1mg 1(mg
EUR 250

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

MBS5785731-5mg 5(mg
EUR 455

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

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Description: Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

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Description: Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

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Description: Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

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Description: Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

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Description: Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

Phosphatase Orphan-2 (Recombinant)

20-abx073747
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Phosphatase Orphan-1 (Recombinant)

20-abx073910
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  • 2 µg
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TR3 Orphan Receptor Antibody

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Recombinant Human Phosphatase Orphan-2

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EUR 240

Recombinant Human Phosphatase Orphan-2

MBS145413-001mg 0.01mg
EUR 310

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MBS145413-1mg 1mg
EUR 5345

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EUR 23725

Recombinant Human Phosphatase Orphan-1

MBS143372-0002mg 0.002mg
EUR 240

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MBS143372-001mg 0.01mg
EUR 310

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Recombinant Human Phosphatase Orphan-1

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Phosphatase Orphan-2 Human Recombinant

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Phosphatase Orphan-1 Human Recombinant

rAP-1537 Inquiry Ask for price

Orphan Receptor Peptide Ligand Library

L-005 150 peptides
EUR 7938

GOAT ANTI HUMAN TR3 ORPHAN RECEPTOR

MBS224277-01mg 0.1mg
EUR 635

GOAT ANTI HUMAN TR3 ORPHAN RECEPTOR

MBS224277-5x01mg 5x0.1mg
EUR 2695

Recombinant Tetraspanning orphan receptor (TOR)

MBS7032068-002mg 0.02mg
EUR 1590

Recombinant Tetraspanning orphan receptor (TOR)

MBS7032068-01mg 0.1mg
EUR 2550

Recombinant Tetraspanning orphan receptor (TOR)

MBS7032068-5x01mg 5x0.1mg
EUR 11430

Anti- Orphan nuclear receptor NR1D1 Antibody

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Anti- Orphan nuclear receptor NR6A1 Antibody

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Anti- Orphan nuclear receptor NR6A1 Antibody

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Anti- Orphan nuclear receptor NR1D2 Antibody

GWB-692AC1 0.05 ml Ask for price

Anti- Orphan nuclear receptor NR1D1 Antibody

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Anti- Orphan nuclear receptor NR6A1 Antibody

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Anti- Orphan nuclear receptor NR5A2 Antibody

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Mouse Anti Respiratory Enteric Orphan Virus

MBS140339-05mg 0.5mg
EUR 440

Mouse Anti Respiratory Enteric Orphan Virus

MBS140339-1mg 1mg
EUR 600

Mouse Anti Respiratory Enteric Orphan Virus

MBS140339-5x1mg 5x1mg
EUR 2375

Anti- Orphan nuclear receptor EAR-2 Antibody

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Anti- Orphan nuclear receptor EAR-2 Antibody

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Anti- Orphan nuclear receptor EAR-2 Antibody

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Anti- Orphan nuclear receptor EAR-2 Antibody

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RAR Related Orphan Receptor Beta (RORB) Antibody

abx027769-400l 400 µl
EUR 518.75

RAR Related Orphan Receptor Beta (RORB) Antibody

abx027769-400ul 400 ul
EUR 627.6

RAR Related Orphan Receptor Beta (RORB) Antibody

abx027769-80l 80 µl
EUR 250

RAR Related Orphan Receptor Beta (RORB) Antibody

abx214131-100l 100 µl
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RAR Related Orphan Receptor Beta (RORB) Antibody

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RAR Related Orphan Receptor Beta (RORB) Antibody

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RAR Related Orphan Receptor Beta (RORB) Antibody

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abx214132-40l 40 µl
EUR 250

Rar-Related Orphan Receptor A (RORA) Antibody

abx115045-100l 100 µl
EUR 612.5

Rar-Related Orphan Receptor A (RORA) Antibody

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Rar-Related Orphan Receptor B (RORB) Antibody

abx115046-100l 100 µl
EUR 612.5

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Rar-Related Orphan Receptor C (RORC) Antibody

abx115047-100l 100 µl
EUR 612.5

Rar-Related Orphan Receptor C (RORC) Antibody

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RAR Related Orphan Receptor Beta (RORB) Antibody

abx237388-100g 100 µg
EUR 350

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EUR 610.8

RAR Related Orphan Receptor Gamma (RORG) Protein

20-abx261968
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Anti- RAR-related orphan receptor B Antibody

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RAR Related Orphan Receptor Alpha (RORA) Antibody

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EUR 281.25

RAR Related Orphan Receptor Gamma (RORG) Antibody

abx013514-100g 100 µg
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RAR Related Orphan Receptor Gamma (RORG) Antibody

abx013514-10g 10 µg
EUR 43.75

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abx013514-200g 200 µg
EUR 312.5

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abx005288-100l 100 µl
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RAR Related Orphan Receptor Alpha (RORA) Antibody

20-abx005288
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  • 20 ul
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RAR Related Orphan Receptor Alpha (RORA) Antibody

abx005288-20l 20 µl
EUR 175

RAR Related Orphan Receptor Alpha (RORA) Antibody

abx005288-50l 50 µl
EUR 275

Neuron Derived Orphan Receptor 1 (NOR1) Antibody

abx130085-100l 100 µl
EUR 287.5

Neuron Derived Orphan Receptor 1 (NOR1) Antibody

20-abx130085
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Neuron Derived Orphan Receptor 1 (NOR1) Antibody

abx130085-1ml 1 ml
EUR 812.5

Neuron Derived Orphan Receptor 1 (NOR1) Antibody

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EUR 362.5

Neuron Derived Orphan Receptor 1 (NOR1) Antibody

20-abx173779
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  • 200 ug
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Neuron Derived Orphan Receptor 1 (NOR1) Antibody

abx173779-1ml 1 ml
EUR 850

RAR Related Orphan Receptor Alpha (RORA) Antibody

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EUR 362.5

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RAR Related Orphan Receptor Alpha (RORA) Antibody

abx338779-20g 20 µg
EUR 162.5

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EUR 250

RAR Related Orphan Receptor Gamma (RORg) Antibody

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  • 200 ug
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RAR Related Orphan Receptor Gamma (RORg) Antibody

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RAR Related Orphan Receptor Alpha (RORA) Antibody

abx211380-50l 50 µl
EUR 250

Detection of BRAF V600E Mutation in Ganglioglioma and Pilocytic Astrocytoma by Immunohistochemistry and Actual-Time PCR-Based mostly Idylla Take a look at

The BRAF V600E mutation is a vital oncological goal in sure central nervous system (CNS) tumors, for which a attainable software of BRAF-targeted remedy grows repeatedly. Within the current research, we goal to find out the prevalence of BRAF V600E mutations in a collection of ganglioglioma (GG) and pilocytic astrocytoma (PA) instances. Concurrently, we determined to confirm whether or not the mix of totally automated checks-BRAF-VE1 immunohistochemistry (IHC) and Idylla BRAF mutation assay-may be helpful to precisely predict it within the case of specified CNS tumors.

The research included 49 formalin-fixed, paraffin-embedded tissues, of which 15 have been GG and 34 PA. Immunohistochemistry with anti-BRAF V600E (VE1) antibody was carried out on tissue sections utilizing the VentanaBenchMark ULTRA platform. All optimistic or equivocal instances on IHC and chosen adverse ones have been additional assessed utilizing the Idylla BRAF mutation assay coupled with the Idylla platform. The BRAF-VE1 IHC was optimistic in 6 (6/49; 12.3%) and adverse in 39 samples (39/49; 79.6%). The interpretation of immunostaining outcomes was sophisticated in Four instances, of which 1 examined optimistic for the Idylla BRAF mutation assay. Subsequently, the general positivity fee was 14.3%. This included 2 instances of GG and 5 instances of PA. Our research discovered that BRAF V600E mutations are reasonably frequent in PA and GG and that for these tumor entities, IHC VE1 is appropriate for screening functions, however all adverse, equivocal, and weak optimistic instances must be additional examined with molecular biology strategies, of which the Idylla system appears to be a promising instrument.

Institution of an SYBR Inexperienced-based real-time PCR assay for porcine circovirus sort Four detection

Porcine circovirus 4 (PCV4) is a novel circovirus first found in China in April 2019. Right here, we established an SYBR Inexperienced I-based real-time PCR for quantitative detection of PCV4. A pair of particular primers was designed based mostly on the conserved area of Cap of PCV4. The usual curve of the established real-time PCR assay confirmed a very good linear relationship. The sensitivity of the established real-time PCR was 100 occasions larger than that of typical PCR, and the detection restrict of the assay was 3 × 101 copies. There was no cross-reactivity with different swine DNA viruses, displaying good specificity.

The intra-group variation coefficient was 0.37-0.78%, and the inter-group variation coefficient was 0.57-0.94%, indicating that the assay has good repeatability. Furthermore, the evaluation of scientific samples confirmed that the optimistic detection fee of PCV4 was 10.71% (18/168), whereas that of typical PCR was 8.93% (15/168). Curiously, co-infection with PCV2 or PCV3, or each PCV2 and PCV3, was additionally detected. In conclusion, the established SYBR Inexperienced I-based real-time PCR could also be an economical and fast methodology for PCV4 scientific analysis.

Orphan GPCR SP9155 agonist P550 (mouse, rat)

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Description: Orphan GPCR SP9155 agonist P550 (mouse, rat)

Orphan GPCR SP9155 agonist P550 (mouse, rat)

T40685-5mg 5mg Ask for price
Description: Orphan GPCR SP9155 agonist P550 (mouse, rat)

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

MBS5785731-10mg 10(mg
EUR 645

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

MBS5785731-1mg 1(mg
EUR 250

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

MBS5785731-5mg 5(mg
EUR 455

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

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Description: Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

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Description: Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

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Description: Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

T40685L-50mg 50mg Ask for price
Description: Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

T40685L-5mg 5mg Ask for price
Description: Orphan GPCR SP9155 agonist P550 (mouse, rat) acetate

Phosphatase Orphan-2 (Recombinant)

20-abx073747
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  • 2 µg
  • 10 ug
  • 1 mg

Phosphatase Orphan-1 (Recombinant)

20-abx073910
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  • 2 µg
  • 10 ug
  • 1 mg

TR3 Orphan Receptor Antibody

GWB-Q00589 0.1 mg Ask for price

Recombinant Human Phosphatase Orphan-2

MBS145413-0002mg 0.002mg
EUR 240

Recombinant Human Phosphatase Orphan-2

MBS145413-001mg 0.01mg
EUR 310

Recombinant Human Phosphatase Orphan-2

MBS145413-1mg 1mg
EUR 5345

Recombinant Human Phosphatase Orphan-2

MBS145413-5x1mg 5x1mg
EUR 23725

Recombinant Human Phosphatase Orphan-1

MBS143372-0002mg 0.002mg
EUR 240

Recombinant Human Phosphatase Orphan-1

MBS143372-001mg 0.01mg
EUR 310

Recombinant Human Phosphatase Orphan-1

MBS143372-1mg 1mg
EUR 6145

Recombinant Human Phosphatase Orphan-1

MBS143372-5x1mg 5x1mg
EUR 27325

Phosphatase Orphan-2 Human Recombinant

rAP-1526 Inquiry Ask for price

Phosphatase Orphan-1 Human Recombinant

rAP-1537 Inquiry Ask for price

Orphan Receptor Peptide Ligand Library

L-005 150 peptides
EUR 7938

GOAT ANTI HUMAN TR3 ORPHAN RECEPTOR

MBS224277-01mg 0.1mg
EUR 635

GOAT ANTI HUMAN TR3 ORPHAN RECEPTOR

MBS224277-5x01mg 5x0.1mg
EUR 2695

Recombinant Tetraspanning orphan receptor (TOR)

MBS7032068-002mg 0.02mg
EUR 1590

Recombinant Tetraspanning orphan receptor (TOR)

MBS7032068-01mg 0.1mg
EUR 2550

Recombinant Tetraspanning orphan receptor (TOR)

MBS7032068-5x01mg 5x0.1mg
EUR 11430

Anti- Orphan nuclear receptor NR1D1 Antibody

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Anti- Orphan nuclear receptor NR1D1 Antibody

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Anti- Orphan nuclear receptor NR6A1 Antibody

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Anti- Orphan nuclear receptor NR6A1 Antibody

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Anti- Orphan nuclear receptor NR1D2 Antibody

GWB-692AC1 0.05 ml Ask for price

Anti- Orphan nuclear receptor NR1D1 Antibody

GWB-797370 0.05 ml Ask for price

Anti- Orphan nuclear receptor NR6A1 Antibody

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Anti- Orphan nuclear receptor NR5A2 Antibody

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Mouse Anti Respiratory Enteric Orphan Virus

MBS140339-05mg 0.5mg
EUR 440

Mouse Anti Respiratory Enteric Orphan Virus

MBS140339-1mg 1mg
EUR 600

Mouse Anti Respiratory Enteric Orphan Virus

MBS140339-5x1mg 5x1mg
EUR 2375

Anti- Orphan nuclear receptor EAR-2 Antibody

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Anti- Orphan nuclear receptor EAR-2 Antibody

GWB-B9F321 0.05 ml Ask for price

Anti- Orphan nuclear receptor EAR-2 Antibody

GWB-D37B4B 0.05 ml Ask for price

Anti- Orphan nuclear receptor EAR-2 Antibody

GWB-835BD4 0.05 ml Ask for price

RAR Related Orphan Receptor Beta (RORB) Antibody

abx027769-400l 400 µl
EUR 518.75

RAR Related Orphan Receptor Beta (RORB) Antibody

abx027769-400ul 400 ul
EUR 627.6

RAR Related Orphan Receptor Beta (RORB) Antibody

abx027769-80l 80 µl
EUR 250

RAR Related Orphan Receptor Beta (RORB) Antibody

abx214131-100l 100 µl
EUR 350

RAR Related Orphan Receptor Beta (RORB) Antibody

20-abx214131
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  • 100 ul

RAR Related Orphan Receptor Beta (RORB) Antibody

abx214131-200l 200 µl Ask for price

RAR Related Orphan Receptor Beta (RORB) Antibody

abx214131-40l 40 µl
EUR 250

RAR Related Orphan Receptor Beta (RORB) Antibody

abx214132-100l 100 µl
EUR 350

RAR Related Orphan Receptor Beta (RORB) Antibody

20-abx214132
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  • 50 ul
  • 100 ul

RAR Related Orphan Receptor Beta (RORB) Antibody

abx214132-200l 200 µl Ask for price

RAR Related Orphan Receptor Beta (RORB) Antibody

abx214132-40l 40 µl
EUR 250

Rar-Related Orphan Receptor A (RORA) Antibody

abx115045-100l 100 µl
EUR 612.5

Rar-Related Orphan Receptor A (RORA) Antibody

20-abx115045
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  • 50 ul
  • 150 ul

Rar-Related Orphan Receptor B (RORB) Antibody

abx115046-100l 100 µl
EUR 612.5

Rar-Related Orphan Receptor B (RORB) Antibody

20-abx115046
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  • 50 ul
  • 150 ul

Rar-Related Orphan Receptor C (RORC) Antibody

abx115047-100l 100 µl
EUR 612.5

Rar-Related Orphan Receptor C (RORC) Antibody

20-abx115047
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  • 50 ul
  • 150 ul

RAR Related Orphan Receptor Beta (RORB) Antibody

abx237388-100g 100 µg
EUR 350

RAR Related Orphan Receptor Beta (RORB) Antibody

abx237388-100ug 100 ug
EUR 610.8

RAR Related Orphan Receptor Gamma (RORG) Protein

20-abx261968
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  • 5 ug
  • 20 ug
  • 1 mg

Anti- RAR-related orphan receptor B Antibody

GWB-DA40DA 0.05 ml Ask for price

Anti- RAR-related orphan receptor B Antibody

GWB-B1C150 0.05 ml Ask for price

Anti- RAR-related orphan receptor B Antibody

GWB-B781E5 0.05 ml Ask for price

RAR Related Orphan Receptor Alpha (RORA) Antibody

abx025951-400l 400 µl
EUR 518.75

RAR Related Orphan Receptor Alpha (RORA) Antibody

abx025951-400ul 400 ul
EUR 627.6

RAR Related Orphan Receptor Alpha (RORA) Antibody

abx025951-80l 80 µl
EUR 281.25

RAR Related Orphan Receptor Gamma (RORG) Antibody

abx013514-100g 100 µg
EUR 237.5

RAR Related Orphan Receptor Gamma (RORG) Antibody

20-abx013514
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  • 10 ug
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  • 300 µg

RAR Related Orphan Receptor Gamma (RORG) Antibody

abx013514-10g 10 µg
EUR 43.75

RAR Related Orphan Receptor Gamma (RORG) Antibody

abx013514-200g 200 µg
EUR 312.5

RAR Related Orphan Receptor Alpha (RORA) Antibody

abx005288-100l 100 µl
EUR 400

RAR Related Orphan Receptor Alpha (RORA) Antibody

20-abx005288
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  • 20 ul
  • 50 ul
  • 100 ul
  • 200 ul

RAR Related Orphan Receptor Alpha (RORA) Antibody

abx005288-20l 20 µl
EUR 175

RAR Related Orphan Receptor Alpha (RORA) Antibody

abx005288-50l 50 µl
EUR 275

Neuron Derived Orphan Receptor 1 (NOR1) Antibody

abx130085-100l 100 µl
EUR 287.5

Neuron Derived Orphan Receptor 1 (NOR1) Antibody

20-abx130085
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  • 10 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Neuron Derived Orphan Receptor 1 (NOR1) Antibody

abx130085-1ml 1 ml
EUR 812.5

Neuron Derived Orphan Receptor 1 (NOR1) Antibody

abx130085-200l 200 µl
EUR 362.5

Neuron Derived Orphan Receptor 1 (NOR1) Antibody

20-abx173779
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  • 200 ug
  • 1 mg

Neuron Derived Orphan Receptor 1 (NOR1) Antibody

abx173779-1ml 1 ml
EUR 850

RAR Related Orphan Receptor Alpha (RORA) Antibody

abx338779-100g 100 µg
EUR 362.5

RAR Related Orphan Receptor Alpha (RORA) Antibody

20-abx338779
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 20 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

RAR Related Orphan Receptor Alpha (RORA) Antibody

abx338779-20g 20 µg
EUR 162.5

RAR Related Orphan Receptor Alpha (RORA) Antibody

abx338779-50g 50 µg
EUR 250

RAR Related Orphan Receptor Gamma (RORg) Antibody

20-abx178212
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  • Ask for price
  • 200 ug
  • 1 mg

RAR Related Orphan Receptor Gamma (RORg) Antibody

abx178212-96tests 96 tests
EUR 287.5

RAR Related Orphan Receptor Gamma (RORG) Antibody

abx217274-100tests 100 tests Ask for price

RAR Related Orphan Receptor Gamma (RORG) Antibody

abx217274-200tests 200 tests Ask for price

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